GM01561
LCL from B-Lymphocyte
Description:
TRANSLOCATED CHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Family Member
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1
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Relation to Proband
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parent
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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46,XX,t(4;11)(4pter>4q23::11q11>11qter;11pter>11q11::4q23>4qter)[8]/45,X,t(4;11)(4pter>4q23::11q11>11qter;11pter>11q11::4q23>4qter),add(19)(?::p13.3>qter)[2]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Cytogenetics |
Chromosome 11: TRANSLOCATION Breakpoint 11q11 t(4;11)11q11 |
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Chromosome 4: TRANSLOCATION Breakpoint 4q23 t(4;11)4q23 |
| Remarks |
27% of cells have 19p+; HLA type:A10, Aw24,Bw35,(B12); see GM00380 Fibroblast |
| Sanford J, Kim BW, Deaven LL, Jones C, Higgins MJ, Nowak NJ, Shows TB, A human chromosome 11 NotI end clone library. Genomics15:653-8 1993 |
| PubMed ID: 8468060 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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