GM01603
Fibroblast from Skin, Unspecified
Description:
APPARENTLY HEALTHY FETAL TISSUE
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Unspecified
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Race
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Black/African American
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis and Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
4 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
46,XY; skin biopsy; same patient as GM01604A; 8% of cells show random chromosome loss |
| Gray SJ, Gerhardt J, Doerfler W, Small LE, Fanning E, An origin of DNA replication in the promoter region of the human fragile X mental retardation (FMR1) gene Molecular and cellular biology27:426-37 2006 |
| PubMed ID: 17101793 |
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| Karlsson C, Paulsson Y, Age related induction of platelet-derived growth factor A-chain mRNA in normal human fibroblasts. J Cell Physiol158:256-62 1994 |
| PubMed ID: 8106562 |
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| Landas S, Foucar K, Sando GN, Ellefson R, Hamilton HE, Adult Niemann-Pick disease masquerading as sea blue histiocyte syndrome: report of a case confirmed by lipid analysis and enzyme assays. Am J Hematol20:391-400 1985 |
| PubMed ID: 4073013 |
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| Ben-Ishai R, Sharon R, Rothman M, Miskin R, DNA repair and induction of plasminogen activator in human fetal cells treated with ultraviolet light. Carcinogenesis5:357-62 1984 |
| PubMed ID: 6538462 |
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| Lawrence DA, Pircher R, Kryceve-Martinerie C, Jullien P, Normal embryo fibroblasts release transforming growth factors in a latent form. J Cell Physiol121:184-8 1984 |
| PubMed ID: 6090474 |
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| Van Keuren, Proteins affected by chromosome 21 and ageing in vitro (from Gene Expression in Normal and Transformed Cells, Plenum Pub Corp; Celis & Bravo, Eds) "Gene Exp in Norm & Trans Cells"1983,pp349:184-8 1983 |
| PubMed ID: 6090474 |
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| Nocentini S, Effects of aphidicolin on the recovery of ribosomal RNA synthesis and on the repair of potentially lethal damage in UV irradiated simian and human cells. Biochem Biophys Res Commun109:603-11 1982 |
| PubMed ID: 6818964 |
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| Sando GN, Henke VL, Recognition and receptor-mediated endocytosis of the lysosomal acid lipase secreted by cultured human fibroblasts. J Lipid Res23:114-23 1982 |
| PubMed ID: 7057100 |
| Passage Frozen |
4 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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