GM01604
Fibroblast from Lung, Lung
Description:
APPARENTLY HEALTHY FETAL TISSUE
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Lung, Lung
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Race
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Black/African American
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis and Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
4 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
46,XY; lung biopsy; same patient as GM01603B |
| Mukhopadhyay S, Clark DR, Watson NB, Zacharias W, McGregor WG, REV1 accumulates in DNA damage-induced nuclear foci in human cells and is implicated in mutagenesis by benzo[a]pyrenediolepoxide Nucleic acids research32:5820-6 2004 |
| PubMed ID: 15523096 |
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| Clark DR, Zacharias W, Panaitescu L, McGregor WG, Ribozyme-mediated REV1 inhibition reduces the frequency of UV-induced mutations in the human HPRT gene. Nucleic Acids Res31(17):4981-8 2003 |
| PubMed ID: 12930947 |
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| Fitch ME, Cross IV, Ford JM, p53 responsive nucleotide excision repair gene products p48 and XPC, but not p53, localize to sites of UV-irradiation-induced DNA damage, in vivo. Carcinogenesis24(5):843-50 2003 |
| PubMed ID: 12771027 |
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| Fitch ME, Cross IV, Turner SJ, Adimoolam S, Lin CX, Williams KG, Ford JM, The DDB2 nucleotide excision repair gene product p48 enhances global genomic repair in p53 deficient human fibroblasts DNA repair2:819-26 2003 |
| PubMed ID: 12826282 |
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| Glockzin S, Ogi FX, Hengstermann A, Scheffner M, Blattner C, Involvement of the DNA repair protein hHR23 in p53 degradation. Mol Cell Biol23(24):8960-9 2003 |
| PubMed ID: 14645509 |
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| Cordeiro-Stone M, Frank A, Bryant M, Oguejiofor I, Hatch SB, McDaniel LD, Kaufmann WK, DNA damage responses protect xeroderma pigmentosum variant from UVC-induced clastogenesis. Carcinogenesis23(6):959-65 2002 |
| PubMed ID: 12082017 |
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| Meira LB, Graham JM Jr, Greenberg CR, Busch DB, Doughty AT, Ziffer DW, Coleman DM, Savre-Train I, Friedberg EC, Manitoba aboriginal kindred with original cerebro-oculo- facio-skeletal syndrome has a mutation in the Cockayne syndrome group B (CSB) gene. Am J Hum Genet66(4):1221-8 2000 |
| PubMed ID: 10739753 |
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| Ouellette MM, McDaniel LD, Wright WE, Shay JW, Schultz RA, The establishment of telomerase-immortalized cell lines representing human
chromosome instability syndromes. Hum Mol Genet9(3):403-11 2000 |
| PubMed ID: 10655550 |
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| Bottles KD, Laszik Z, Morrissey JH, Kinasewitz GT, Tissue factor expression in mesothelial cells: induction both in vivo and in vitro. Am J Respir Cell Mol Biol17:164-72 1997 |
| PubMed ID: 9271304 |
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| Jesudasan RA, Rahman RA, Chandrashekharappa S, Evans GA, Srivatsan ES, Deletion and translocation of chromosome 11q13 sequences in cervical carcinoma cell lines [see comments] Am J Hum Genet56:705-15 1995 |
| PubMed ID: 7887426 |
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| Bloem LJ, Chen L, Konigsberg WH, Bach R, Serum stimulation of quiescent human fibroblasts induces the synthesis of tissue factor mRNA followed by the appearance of tissue factor antigen and procoagulant activity. J Cell Physiol139:418-23 1989 |
| PubMed ID: 2715194 |
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| Bach R, Konigsberg WH, Nemerson Y, Human tissue factor contains thioester-linked palmitate and stearate on the cytoplasmic half-cystine. Biochemistry27:4227-4231 1988 |
| PubMed ID: 3166978 |
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| Hutchins JT, Reading CL, Giavazzi R, Hoaglund J, Jessup JM, Distribution of mono-, di, and tri-O-acetylated sialic acids in normal and neoplastic colon. Cancer Res48:483-9 1988 |
| PubMed ID: 3335016 |
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| Finlay CA, Rosner BA, Miller JE, Wallace JM, Cristofalo VJ, Enhanced proliferation of WI38 cells in the presence of glucocorticoid- conditioned medium. Exp Cell Res156:462-70 1985 |
| PubMed ID: 3967687 |
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| Kato G, Suzuki Y, Phosphomannosyl receptor in bovine and human tissues determined by a sensitive radioimmunoassay method. J Appl Biochem7:282-8 1985 |
| PubMed ID: 3005223 |
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| Ben-Ishai R, Sharon R, Rothman M, Miskin R, DNA repair and induction of plasminogen activator in human fetal cells treated with ultraviolet light. Carcinogenesis5:357-62 1984 |
| PubMed ID: 6538462 |
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| Jaffe EA, Ruggiero JT, Leung LK, Doyle MJ, McKeown-Longo PJ, Mosher DF, Cultured human fibroblasts synthesize and secrete thrombospondin and incorporate it into extracellular matrix. Proc Natl Acad Sci U S A80:998-1002 1983 |
| PubMed ID: 6341993 |
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| Der CJ, Stanbridge EJ, A tumor-specific membrane phosphoprotein marker in human cell hybrids. Cell26:429-38 1981 |
| PubMed ID: 7326746 |
| Passage Frozen |
4 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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