GM02132
LCL from B-Lymphocyte
Description:
MYELOMA, MULTIPLE AMYLOIDOSIS, PRIMARY, INCLUDED; AL, INCLUDED
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Heritable Diseases |
| Class |
Other Disorders of Known Biochemistry |
|
Biopsy Source
|
Peripheral vein
|
|
Cell Type
|
B-Lymphocyte
|
|
Tissue Type
|
Blood
|
|
Transformant
|
Epstein-Barr Virus
|
|
Sample Source
|
LCL from B-Lymphocyte
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
Line RPMI8226; ATCC CCL155; IgG Lambda type light chain secreting; G6PD = A; negative for reverse transcriptase; modal no. of 65 chromosomes; range = 40 to 78; low TdT activity; negative for EBV and EBNA |
| Agabiti SS, Li J, Dong W, Poe MM, Wiemer AJ, Regulation of the Notch-ATM-abl axis by geranylgeranyl diphosphate synthase inhibition Cell death & disease10:733 2019 |
| PubMed ID: 31570763 |
| |
| Klobeck HG, Zimmer FJ, Combriato G, Zachau HG, Linking of the human immunoglobulin VK and JKCK regions by chromosomal walking. Nucleic Acids Res15:9655-65 1987 |
| PubMed ID: 3122178 |
| |
| Sanford JA, Stubblefield E, General protocol for microcell-mediated chromosome transfer. Somat Cell Mol Genet13:279-84 1987 |
| PubMed ID: 3299749 |
| |
| Klobeck HG, Zachau HG, The human CK gene segment and the kappa deleting element are closely linked. Nucleic Acids Res14:4591-603 1986 |
| PubMed ID: 3086844 |
| |
| Mocikat R, Falkner FG, Mertz R, Zachau HG, Upstream regulatory sequences of immunoglobulin genes are recognized by nuclear proteins which also bind to other gene regions. Nucleic Acids Res14:8829-44 1986 |
| PubMed ID: 3786139 |
| |
| Goldstein M, Hoxie J, Zembryki D, Matthews D, Levinson AI, Phenotypic and functional analysis of B cell lines from patients with multiple myeloma. Blood66:444-6 1985 |
| PubMed ID: 3893568 |
| |
| Sutherland BM, Bennett PV, Freeman AG, Moore SP, Strickland PT, Transformation of human cells by DNAs ineffective in transformation of NIH 3T3 cells. Proc Natl Acad Sci U S A82:2399-403 1985 |
| PubMed ID: 3857589 |
| |
| Lane MA, Sainten A, Cooper GM, Stage-specific transforming genes of human and mouse B- and T- lymphocyte neoplasms. Cell28:873-80 1982 |
| PubMed ID: 6284377 |
| |
| Matsuoka Y, Moore GE, Yagi Y, Pressman D, Production of free light chains of immunoglobulin by a hematopoietic cell line derived from a patient with multiple myeloma. Proc Soc Exp Biol Med125:1246-50 1967 |
| PubMed ID: 6042436 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
|
|