Description:
CHROMOSOME DELETION
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Chromosome Abnormalities |
Cell Type
|
Fibroblast
|
Transformant
|
Untransformed
|
Race
|
White
|
Relation to Proband
|
proband
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
ISCN
|
46,XY,del(18)(pter>q21:)
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Passage Frozen |
2 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
|
Cytogenetics |
Chromosome 18: DELETION Aneuploid Segment (-)18q21>18qter |
Nishisho I, Tateishi H, Motomura K, Miki T, Yoshida MC, Ikeuchi T, Yamamoto K, Okazaki M, Takai S, Mori T, Assignment of a polymorphic locus of OS-4(D18S5) DNA segment to human chromosome region 18q21.3----qter. Jinrui Idengaku Zasshi32:1-7 1987 |
PubMed ID: 2886683 |
Passage Frozen |
2 |
Split Ratio |
1:4 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
|