GM03523
Fibroblast from Skin, Neck
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Neck
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Neck
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY,t(3;11)(3pter>3q24::11p15>11pter;11qter>11p15::3q24>3qter)[15]/46,XY[35]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
7 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
Skin biopsy (right neck); 46,XY; 8% of cells show random chromosome loss |
| Bersini S, Schulte R, Huang L, Tsai H, Hetzer MW, Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome eLife9: 2019 |
| PubMed ID: 32896271 |
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| Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19: 2018 |
| PubMed ID: 30567591 |
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| Abdullah, K.M., Abdullah, A., Johnson, M.L., Bilski, J.J., Petry, K., Redmer, D.A., Reynolds, L.P., and Grazul-Bilska, A.T., Effects of Aloe vera on Gap Junctional Intercellular Communication and Proliferation of Human Diabetic and Nondiabetic Skin Fibroblasts J Alternative and Complementary Med9(5):711-718 2003 |
| PubMed ID: 14629848 |
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| Miller JW, Urbinati CR, Teng-Umnuay P, Stenberg MG, Byrne BJ, Thornton CA,
Swanson MS, Recruitment of human muscleblind proteins to (CUG)(n) expansions associated with
myotonic dystrophy. EMBO J19(17):4439-48 2000 |
| PubMed ID: 10970838 |
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| Michalowski S, Miller JW, Urbinati CR, Paliouras M, Swanson MS, Griffith J, Visualization of double-stranded RNAs from the myotonic dystrophy protein kinase gene and interactions with CUG-binding protein. Nucleic Acids Res27(17):3534-42 1999 |
| PubMed ID: 10446244 |
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| Peterson C, Ratan RR, Shelanski ML, Goldman JE, Altered response of fibroblasts from aged and Alzheimer donors to drugs that elevate cytosolic free calcium. Neurobiol Aging9:261-6 1988 |
| PubMed ID: 3398992 |
| Passage Frozen |
7 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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