GM03529
Fibroblast from Skin, Thigh
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Thigh
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Thigh
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Race
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Black/African American
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis and Case history
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ISCN
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46,XY[19]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
7 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Skin biopsy (left thigh); 46,XY; 6% of cells show random chromosome loss/gain and 4% show random chromosomal aberrations |
| Cha Y, Leblanc P, Hong YJ, Kim KS, Integrative analysis of mitochondrial metabolic dynamics in reprogramming human fibroblast cells STAR protocols3:101401 2022 |
| PubMed ID: 35600932 |
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| Kuo SH, Tasset I, Cheng MM, Diaz A, Pan MK, Lieberman OJ, Hutten SJ, Alcalay RN, Kim S, Ximénez-Embún P, Fan L, Kim D, Ko HS, Yacoubian T, Kanter E, Liu L, Tang G, Muñoz J, Sardi SP, Li A, Gan L, Cuervo AM, Sulzer D, Mutant glucocerebrosidase impairs a-synuclein degradation by blockade of chaperone-mediated autophagy Science advances8:eabm6393 2022 |
| PubMed ID: 35138901 |
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| Cha Y, Kim T, Jeon J, Jang Y, Kim PB, Lopes C, Leblanc P, Cohen BM, Kim KS, SIRT2 regulates mitochondrial dynamics and reprogramming via MEK1-ERK-DRP1 and AKT1-DRP1 axes Cell reports37:110155 2021 |
| PubMed ID: 34965411 |
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| Bersini S, Schulte R, Huang L, Tsai H, Hetzer MW, Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome eLife9:110155 2019 |
| PubMed ID: 32896271 |
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| Song B, Cha Y, Ko S, Jeon J, Lee N, Seo H, Park KJ, Lee IH, Lopes C, Feitosa M, Luna MJ, Jung JH, Kim J, Hwang D, Cohen B, Teicher M, Leblanc P, Carter B, Kordower JH, Bolshakov VY, Kong SW, Schweitzer JS, Kim KS, Human Autologous iPSC-Derived Dopaminergic Progenitors Restore Motor Function in Parkinson's Disease Models The Journal of clinical investigation9:110155 2019 |
| PubMed ID: 31714896 |
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| Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19:110155 2018 |
| PubMed ID: 30567591 |
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| Hebert L, Pandey S, Wang E, Commitment to cell death is signaled by the appearance of a terminin protein of 30 kDa. Exp Cell Res210:10-8 1994 |
| PubMed ID: 8269985 |
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| Karlsson C, Paulsson Y, Age related induction of platelet-derived growth factor A-chain mRNA in normal human fibroblasts. J Cell Physiol158:256-62 1994 |
| PubMed ID: 8106562 |
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| Eleftheriou CS, Trakas N, Kokla A, Tzartos SJ, A group of three fibroblast secreted polypeptides suppressed by cellular ageing and interferon-gamma. Biochim Biophys Acta1180:304-12 1993 |
| PubMed ID: 7678507 |
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| Pienta KJ, Getzenberg RH, Coffey DS, Characterization of nuclear morphology and nuclear matrices in ageing human fibroblasts. Mech Ageing Dev62:13-24 1992 |
| PubMed ID: 1560681 |
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| Sester U, Sawada M, Wang E, Purification and biochemical characterization of statin, a nonproliferation specific protein from rat liver. J Biol Chem265:19966-72 1990 |
| PubMed ID: 2246273 |
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| Ethier MF, Hickler RB, Dobson JG Jr, Aging increases adenosine and inosine release by human fibroblast cultures. Mech Ageing Dev50:159-68 1989 |
| PubMed ID: 2601414 |
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| Ching G, Wang E, Absence of three secreted proteins and presence of a 57-kDa protein related to irreversible arrest of cell growth. Proc Natl Acad Sci U S A85:151-5 1988 |
| PubMed ID: 3422412 |
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| Connolly JA, Sarabia VE, Kelvin DJ, Wang E, The disappearance of a cyclin-like protein and the appearance of statin is correlated with the onset of differentiation during myogenesis in vitro. Exp Cell Res174:461-71 1988 |
| PubMed ID: 2892689 |
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| Tamm I, Kikuchi T, Wang E, Pfeffer LM, Growth rate of control and beta-interferon-treated human fibroblast populations over the course of their in vitro life span. Cancer Res44:2291-6 1984 |
| PubMed ID: 6722770 |
| Passage Frozen |
7 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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