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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Hybridoma
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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Species
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Rattus norvegicus
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Common Name
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brown or Norway rat
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Remarks
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| Remarks |
Lewis rat spleen cells fused with mouse myeloma P3X63Ag8; line D4,69; produces rat IgG2c, Kappa and mouse IgG1, Kappa; antigen specificity: RT1-N.A; medium contains 4.5g glucose per liter |
| Smilek DE, Boyd HC, Wilson DB, Zmijewski CM, Fitch FW, McKearn TJ, Monoclonal rat anti-major histocompatibility complex antibodies display specificity for rat, mouse, and human target cells. J Exp Med151:1139-50 1980 |
| PubMed ID: 6768831 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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