Description:
MYELOMA (PLASMACYTOMA) CELL LINES FOR HYBRIDOMAS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Myeloma
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Family Member
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1
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Confirmation
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Clinical summary/Case history
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Mouse myeloma, line SP2/0; HPRT deficient; non-immunoglobulin producing; medium contains 4.5g glucose per liter |
| Chatterjee D, Mandal C, Sarkar PK, Development and characterization of five monoclonal antibodies against neuronal cell surface antigens--evaluation of their use in cell separation by affinity chromatography. J Neuroimmunol15:251-62 1987 |
| PubMed ID: 3298317 |
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| Shulman M, Wilde CD, Kohler G, A better cell line for making hybridomas secreting specific antibodies. Nature276:269-70 1978 |
| PubMed ID: 714156 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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