Description:
MYELOMA (PLASMACYTOMA) CELL LINES FOR HYBRIDOMAS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Myeloma
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Confirmation
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Clinical summary/Case history
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Mouse myeloma; line P3X63Ag8653; HPRT deficient; non-immunoglobulin producing; derived from P3 by Rajewsky; medium contains 4.5g glucose per liter |
| Brown PJ, Juliano RL, Monoclonal antibodies to distinctive epitopes on the alpha and beta subunits of the fibronectin receptor. Exp Cell Res177:303-18 1988 |
| PubMed ID: 2455654 |
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| Stamatoglou SC, Keller JM, Correlation between cell substrate attachment in vitro and cell surface heparan sulfate affinity for fibronectin and collagen. J Cell Biol96:1820-3 1983 |
| PubMed ID: 6222058 |
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| Telakowski-Hopkins CA, Lu AY, Pickett CB, Isolation and characterization of monoclonal antibodies against rat liver epoxide hydrolase. Arch Biochem Biophys221:79-88 1983 |
| PubMed ID: 6402988 |
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| Kearney JF, Radbruch A, Liesegang B, Rajewsky K, A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J Immunol123:1548-50 1979 |
| PubMed ID: 113458 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Percent O2 |
AMBIENT |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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