Description:
MYELOMA (PLASMACYTOMA) CELL LINES FOR HYBRIDOMAS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Myeloma
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Confirmation
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Clinical summary/Case history
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis |
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| Remarks |
BALB/C mouse myeloma; line P3X63Ag8; secretes IgG1(K) (MOPC21); HPRT deficient; original Milstein line; medium contains 4.5g glucose per liter |
| Croce CM, Shander M, Martinis J, Cicurel L, D'Ancona GG, Dolby TW, Koprowski H, Chromosomal location of the genes for human immunoglobulin heavy chains. Proc Natl Acad Sci U S A76:3416-9 1979 |
| PubMed ID: 114999 |
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| Kennett RH, Gilbert F, Hybrid myelomas producing antibodies against a human neuroblastoma antigen present on fetal brain. Science203:1120-1 1979 |
| PubMed ID: 424740 |
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| Lemke H, Hammerling GJ, Hohmann C, Rajewsky K, Hybrid cell lines secreting monoclonal antibody specific for major histocompatibility antigens of the mouse. Nature271:249-51 1978 |
| PubMed ID: 74805 |
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| Kohler G, Pearson T, Milstein C, Fusion of T and B cells. Somatic Cell Genet3:303-12 1977 |
| PubMed ID: 305123 |
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| Koprowski H, Gerhard W, Croce CM, Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells. Proc Natl Acad Sci U S A74:2985-8 1977 |
| PubMed ID: 268647 |
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| Kohler G, Milstein C, Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol6:511-9 1976 |
| PubMed ID: 825377 |
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| Kohler G, Milstein C, Continuous cultures of fused cells secreting antibody of predefined specificity. Nature256:495-7 1975 |
| PubMed ID: 1172191 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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