Description:
MYELOMA (PLASMACYTOMA) CELL LINES FOR HYBRIDOMAS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Myeloma
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Confirmation
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Clinical summary/Case history
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis |
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| Remarks |
Mouse myeloma; line 45,6TG1.7; HPRT deficient; produces IgG2(K); derived by Scharff; medium contains 4.5g glucose per liter |
| Franchitto A, Oshima J, Pichierri P, The G2-phase decatenation checkpoint is defective in Werner syndrome cells. Cancer Res63(12):3289-95 2003 |
| PubMed ID: 12810661 |
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| Gefter ML, Margulies DH, Scharff MD, A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells. Somatic Cell Genet3:231-6 1977 |
| PubMed ID: 605383 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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