GM03658
Fibroblast from Skin, Arm
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis and Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
5.66 |
| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
Skin biopsy (left forearm); 46,XY: see GM03657A Lymphoid |
| de Andrade KC, Pinto EM, Zhao T, Zeigler LP, Kim J, Giri N, Haley JS, McReynolds LJ, Florez-Vargas O, Phillips AH, Kriwacki RW, Akinniyi SA, Cohen SB, Emerson MR, Smelser DT, Urban GM, Fridman C, Zambetti GP, Bryan TM, Carey DJ, Garcia CK, Stewart DR, Savage SA, TERT c3150 G > C (pK1050N): a founder Ashkenazi Jewish variant associated with telomere biology disorders NPJ genomic medicine10:46 2025 |
| PubMed ID: 40456748 |
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| Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19:46 2018 |
| PubMed ID: 30567591 |
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| Sims NR, Blass JP, Phosphofructokinase activity in fibroblasts from patients with Alzheimer's disease and age- and sex-matched controls. Metab Brain Dis1:83-90 1986 |
| PubMed ID: 2977422 |
| Passage Frozen |
3 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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