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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Hybridoma
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|
Transformant
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Polyethylene glycol (hybridoma fusion)
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|
Confirmation
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Clinical summary/Case history
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|
Species
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Rattus norvegicus
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Common Name
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brown or Norway rat
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Remarks
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|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Lewis rat spleen cells fused with mouse myeloma SP2/O; line PhK2.13.2; antigen specificity: RT1-N.A; medium contains 4.5g glucose per liter and gamma globulin free horse serum instead of fetal bovine serum |
| Smilek DE, Boyd HC, Wilson DB, Zmijewski CM, Fitch FW, McKearn TJ, Monoclonal rat anti-major histocompatibility complex antibodies display specificity for rat, mouse, and human target cells. J Exp Med151:1139-50 1980 |
| PubMed ID: 6768831 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum |
| Supplement |
- |
|
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