Description:
ISODICENTRIC CHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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46,X,idic(X)(pter>q26::q26>pter)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
8 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Cytogenetics |
Chromosome X: DICENTRIC CHROMOSOME Aneuploid Segment (+)Xpter>Xq26 |
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Chromosome X: DICENTRIC CHROMOSOME Aneuploid Segment (-)Xq26>Xqter |
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Chromosome X: DICENTRIC CHROMOSOME Trisomic Segment Xpter>Xq26 |
| Remarks |
Primary amenorrhea, underdeveloped uterus, bilateral streak ovaries; dic(X) is late replicating |
| Higgins AW, Schueler MG, Willard HF, Chromosome engineering: generation of mono- and dicentric isochromosomes in a somatic cell hybrid system. Chromosoma108:256-65 1999 |
| PubMed ID: 10460414 |
| |
| Just W, Geerkens C, Held KR, Vogel W, Expression of RPS4X in fibroblasts from patients with structural aberrations of the X chromosome. Hum Genet89:240-2 1992 |
| PubMed ID: 1587534 |
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| Riddell DC, Wang HS, Beckett J, Chan A, Holden JJ, Mulligan LM, Phillips MA, Simpson NE, Wrogemann K, Hamerton JL, et al, Regional localization of 18 human X-linked DNA sequences. Cytogenet Cell Genet42:123-8 1986 |
| PubMed ID: 3460742 |
| Passage Frozen |
8 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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