Description:
ISODICENTRIC CHROMOSOME
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Chromosome Abnormalities |
|
Cell Type
|
Fibroblast
|
|
Transformant
|
Untransformed
|
|
Race
|
White
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
|
ISCN
|
46,X,idic(X)(pter>q26::q26>pter)
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| Passage Frozen |
8 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
| |
| Cytogenetics |
Chromosome X: DICENTRIC CHROMOSOME Aneuploid Segment (+)Xpter>Xq26 |
|
Chromosome X: DICENTRIC CHROMOSOME Aneuploid Segment (-)Xq26>Xqter |
|
Chromosome X: DICENTRIC CHROMOSOME Trisomic Segment Xpter>Xq26 |
| Remarks |
Primary amenorrhea, underdeveloped uterus, bilateral streak ovaries; dic(X) is late replicating |
| Higgins AW, Schueler MG, Willard HF, Chromosome engineering: generation of mono- and dicentric isochromosomes in a somatic cell hybrid system. Chromosoma108:256-65 1999 |
| PubMed ID: 10460414 |
| |
| Just W, Geerkens C, Held KR, Vogel W, Expression of RPS4X in fibroblasts from patients with structural aberrations of the X chromosome. Hum Genet89:240-2 1992 |
| PubMed ID: 1587534 |
| |
| Riddell DC, Wang HS, Beckett J, Chan A, Holden JJ, Mulligan LM, Phillips MA, Simpson NE, Wrogemann K, Hamerton JL, et al, Regional localization of 18 human X-linked DNA sequences. Cytogenet Cell Genet42:123-8 1986 |
| PubMed ID: 3460742 |
| Passage Frozen |
8 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|