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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Hybridoma
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
C57Bl6 mouse immunized with cultured human neuroblastoma cells fused with mouse myeloma P3X63Ag8; produces antibody (IgM) against human neuroblastoma & B & null cell leukemias; line PI153/3; antibody binds complement |
| Danner D, Leder P, Role of an RNA cleavage/poly(A) addition site in the production of membrane-bound and secreted IgM mRNA. Proc Natl Acad Sci U S A82:8658-62 1985 |
| PubMed ID: 3936040 |
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| Greaves MF, Verbi W, Kemshead J, Kennett R, A monoclonal antibody identifying a cell surface antigen shared by common acute lymphoblastic leukemias and B lineage cells. Blood56:1141-4 1980 |
| PubMed ID: 6969098 |
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| Momoi M, Kennett RH, Glick MC, A membrane glycoprotein from human neuroblastoma cells isolated with the use of a monoclonal antibody. J Biol Chem255:11914-21 1980 |
| PubMed ID: 6160155 |
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| Kennett RH, Gilbert F, Hybrid myelomas producing antibodies against a human neuroblastoma antigen present on fetal brain. Science203:1120-1 1979 |
| PubMed ID: 424740 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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