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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Hybridoma
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis |
| |
| Remarks |
Balb/C mouse immunized with human B-cell line fused with mouse myeloma P3X63Ag8; produces cytotoxic antibody (IgG2a) against human HLA: A,B,& C; line KE2; medium contains 4.5g glucose per liter |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Percent O2 |
AMBIENT |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
Semi-suspension culture |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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