Description:
MYELOMA (PLASMACYTOMA) CELL LINES FOR HYBRIDOMAS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Myeloma
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Confirmation
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Clinical summary/Case history
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis |
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| Remarks |
Mouse myeloma; line S194; derived from an IgA (alpha, kappa) secreting myeloma which arose after mineral oil induction in a Balb/c mouse; synthesizes heavy & light chain but no immunoglobulin is secreted; resistant to bromodeoxyuridine |
| Hyman R, Ralph P, Sarkar S, Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J Natl Cancer Inst48:173-184 1972 |
| PubMed ID: 4119881 |
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| Horibata K, Harris AW, Mouse myelomas and lymphomas in culture. Exp Cell Res60:61-77 1970 |
| PubMed ID: 5439579 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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