GM05862
Fibroblast from Skin, Embryo
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Biopsy Source
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Embryo
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Embryo
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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73~78,X~XXYYYY,-1,-4,+6,-7,+8,+8,-9,-10,+11,+der(11;17)(A1;A1),-12,del(12)(D3),-13,-14,+15,-16,-17,-18,-19,+2~7mar[cp10]
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| Passage Frozen |
38 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Genbank |
COX1 |
| Remarks |
Mouse line 3T6; fibroblast cell line established by Todaro and Green from Swiss albino mouse embryos; secretes collagen and hyaluronic acid |
| Shackley DC, Haylett A, Whitehurst C, Betts CD, O'Flynn K, Clarke NW, Moore JV, Comparison of the cellular molecular stress responses after treatments used in
bladder cancer. BJU Int90(9):924-32 2002 |
| PubMed ID: 12460358 |
| |
| Hirai M, Kusuda J, Hashimoto K, Assignment of human ADP ribosylation factor (ARF) genes ARF1 and ARF3 to chromosomes 1q42 and 12q13, respectively. Genomics34(2):263-5 1996 |
| PubMed ID: 8661066 |
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| Kusuda J, Tanuma R, Gotoh E, Toyoda A, Hashimoto K, Assignment of a human autoimmune antigen, p80-coilin gene to chromosome 17q21-q23 and of its possible pseudogene to chromosome 14. Hum Genet95:233-4 1995 |
| PubMed ID: 7860074 |
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| Howe JR, Lairmore TC, Veile R, Dou S, Wells SA Jr, Donis-Keller H, Development of a sequence-tagged site for the centromere of chromosome 10: its use in cytogenetic and physical mapping. Hum Genet91:199-204 1993 |
| PubMed ID: 8478002 |
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| Draber P, Stanley P, Isolation and partial characterization of lectin-resistant F9 cells. Somat Cell Mol Genet10:445-54 1984 |
| PubMed ID: 6591444 |
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| Buck DW, Kennett RH, McGarrity G, Monoclonal antibodies specific for cell culture mycoplasmas. In Vitro18:377-81 1982 |
| PubMed ID: 6811415 |
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| Green H, Goldberg B, Todaro GJ, Differentiated cell types and the regulation of collagen synthesis. Nature212:631-3 1966 |
| PubMed ID: 5971697 |
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| Todaro, Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J Cell Biol17:299 (1963):631-3 1963 |
| PubMed ID: 5971697 |
| Passage Frozen |
38 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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