GM06819
LCL from B-Lymphocyte
Description:
HLA HISTOCOMPATIBILITY TYPE: 142800 THROUGH 142860 & 142880
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Heritable Diseases |
|
Biopsy Source
|
Peripheral vein
|
|
Cell Type
|
B-Lymphocyte
|
|
Tissue Type
|
Blood
|
|
Transformant
|
Epstein-Barr Virus
|
|
Sample Source
|
LCL from B-Lymphocyte
|
|
Race
|
White
|
|
Ethnicity
|
DUTCH
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this cell culture gave a positive result with a primer for Yq11, DYS227. |
| |
| Remarks |
Homozygous HLA type: A2,B18,Cw5,Dw7,DR5, & SB2; product of consanguineous (first cousin) marriage; line JVM |
| Brodsky FM, A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes. Immunogenetics19:179-194 1984 |
| PubMed ID: 6200433 |
| dbSNP |
dbSNP ID: 10889 |
| Gene Cards |
HLA-A |
|
HLA-DPA1 |
|
HLA-DRA |
| Gene Ontology |
GO:0005887 integral to plasma membrane |
|
GO:0006955 immune response |
|
GO:0019883 antigen presentation, endogenous antigen |
|
GO:0019884 antigen presentation, exogenous antigen |
|
GO:0019885 antigen processing, endogenous antigen via MHC class I |
|
GO:0019886 antigen processing, exogenous antigen via MHC class II |
|
GO:0030106 MHC class I receptor activity |
|
GO:0045012 MHC class II receptor activity |
| NCBI Gene |
Gene ID:3105 |
|
Gene ID:3113 |
|
Gene ID:3122 |
| NCBI GTR |
142800 MAJOR HISTOCOMPATIBILITY COMPLEX, CLASS I, A; HLA-A |
|
142860 MAJOR HISTOCOMPATIBILITY COMPLEX, CLASS II, DR ALPHA; HLA-DRA |
|
142880 MAJOR HISTOCOMPATIBILITY COMPLEX, CLASS II, DP ALPHA-1; HLA-DPA1 |
| OMIM |
142800 MAJOR HISTOCOMPATIBILITY COMPLEX, CLASS I, A; HLA-A |
|
142860 MAJOR HISTOCOMPATIBILITY COMPLEX, CLASS II, DR ALPHA; HLA-DRA |
|
142880 MAJOR HISTOCOMPATIBILITY COMPLEX, CLASS II, DP ALPHA-1; HLA-DPA1 |
| Omim Description |
HLA-A HISTOCOMPATIBILITY TYPE |
| |
MAJOR HISTOCOMPATIBILITY COMPLEX, CLASS I, A; HLA-A |
| |
HLA-DR HISTOCOMPATIBILITY TYPE; HLA-DRA |
| |
MAJOR HISTOCOMPATIBILITY COMPLEX II, DR ALPHA; HLA-DRA1 |
| |
HLA-SB HISTOCOMPATIBILITY TYPE; HLASB; HLADP |
| |
MAJOR HISTOCOMPATIBILITY COMPLEX, CLASS II, DP ALPHA-1; HLA-DPA1 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
|
|