GM06853
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/RAT SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Family Member
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23
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Rat somatic cell hybrid retaining the human X chromosome
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for Xp22.2, DXS16, and positive results with probes for Xp22.32 (also Yp11.3), MIC2, and Xq28, DXS15. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp22.32, STS, Xq13.3, PGK1, Xq26, HPRT, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 14q32.33, IGHG4. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 16pterp13, D16S36, and 16q13q22.1, MT2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17q21.3q23, MPO. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 21q11, D21S26, and 21q22.1q22.2, D21S17. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. |
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| Remarks |
Human fetal liver fibroblast X rat hepatoma line 7777-14b-AZA; selected in HAT medium; retains the human X chromosome in 100% of cells; isoenzyme analysis was negative for GPI, ADA, and ACO2; culture grows best in suspension |
| Gorski JL, Burright EN, Reyner EL, Goodfellow PN, Burgess DL, Isolation of DNA markers from a region between incontinentia pigmenti 1 (IP1) X-chromosomal translocation breakpoints by a comparative PCR analysis of a radiation hybrid subclone mapping panel. Genomics14:649-56 1992 |
| PubMed ID: 1427891 |
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| Nakamura N, Sugino H, Takahara K, Jin C, Fukushige S, Matsubara K, Endogenous retroviral LTR DNA sequences as markers for individual human chromosomes. Cytogenet Cell Genet57:18-22 1991 |
| PubMed ID: 1713140 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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