GM07298
Somatic cell hybrid
Description:
CHROMOSOME X REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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Human/Chinese hamster hybrid retaining human chromosomes t(X;11), #4, 14, 20, & 21
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for Xp11.21, ALAS2, Xp11.3, DXS1003, and Xp22.32, STS, and gave positive results with primers for Xq12, DXS453, Xq13.3, PGK1, Xq21.3, DXS3, Xq26, HPRT, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for Yq11, DYS227. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 4p15.3, QDPR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 11p13, CAT, and gave a positive result with a primer for 11q21-q22, CLG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 14q11.2, NP. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 20 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 20q12-q13.11, ADA. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 21q22.3, ITGB2. |
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| Remarks |
Line #2.12; human/HPRT-deficient Chinese hamster lung cell line; selected in medium containing azaserine; retains human chromosomes #4 (28%), #14 (92%), #20 (12%), #21 (64%), & t(X;11)(Xqter>cen> 11qter) in 100% of cells |
| Harun RB, Smith KK, Leek JP, Markham AF, Norris A, Morrison JF, Characterization of human SHC p66 cDNA and its processed pseudogene mapping to Xq12-q13.1. Genomics42:349-52 1997 |
| PubMed ID: 9192859 |
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| Boycott KM, Halley GR, Schlessinger D, Bech-Hansen NT, A 2-megabase physical contig incorporating 43 DNA markers on the human X chromosome at p11.23-p11.22 from ZNF21 to DXS255. Genomics33:488-97 1996 |
| PubMed ID: 8661008 |
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| Argeson AC, Druck T, Veronese ML, Knopf JL, Buchberg AM, Huebner K, Siracusa LD, Phospholipase C gamma-2 (Plcg2) and phospholipase C gamma-1 (Plcg1) map to distinct regions in the human and mouse genomes. Genomics25:29-35 1995 |
| PubMed ID: 7774933 |
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| Leek J, Lench N, Maraj B, Bailey A, Carr IM, Andersen S, Cross J, Whelan P, MacLennan KA, Meredith DM, Prostate-specific membrane antigen: evidence for the existence of a second related human gene British journal of cancer72:583-8 1995 |
| PubMed ID: 7669565 |
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| Huebner K, Kastury K, Druck T, Salcini AE, Lanfrancone L, Pelicci G, Lowenstein E, Li W, Park SH, Cannizzaro L, et al, Chromosome locations of genes encoding human signal transduction adapter proteins, Nck (NCK), Shc (SHC1), and Grb2 (GRB2). Genomics22:281-7 1994 |
| PubMed ID: 7806213 |
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| Oaks MK, Hanson JP Jr, O'Malley DP, Molecular cytogenetic mapping of the human melanoma antigen (MAGE) gene family to chromosome region Xq27-qter: implications for MAGE immunotherapy. Cancer Res54:1627-9 1994 |
| PubMed ID: 8137270 |
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| Huebner K, Druck T, LaForgia S, Lasota J, Croce CM, Lanfrancone L, Donti E, Pengue G, La Mantia G, Pelicci PG, et al, Chromosomal localization of four human zinc finger cDNAs. Hum Genet91:217-22 1993 |
| PubMed ID: 8478004 |
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| Kremer EJ, Kalatzis V, Baker E, Callen DF, Sutherland GR, Maliszewski CR, The gene for the human IgA Fc receptor maps to 19q13.4. Hum Genet89:107-8 1992 |
| PubMed ID: 1577457 |
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| Mariyama M, Zheng K, Yang-Feng TL, Reeders ST, Colocalization of the genes for the alpha 3(IV) and alpha 4(IV) chains of type IV collagen to chromosome 2 bands q35-q37. Genomics13:809-13 1992 |
| PubMed ID: 1639407 |
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| Yang-Feng TL, Zheng K, Yu J, Yang BZ, Chen YT, Kao FT, Assignment of the human glycogen debrancher gene to chromosome 1p21. Genomics13:931-4 1992 |
| PubMed ID: 1505983 |
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| Yang-Feng TL, Zheng K, Kopatz I, Naiman T, Canaani D, Mapping of the human casein kinase II catalytic subunit genes: two loci carrying the homologous sequences for the alpha subunit. Nucleic Acids Res19:7125-9 1991 |
| PubMed ID: 1766873 |
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| Chen YT, Rettig WJ, Yenamandra AK, Kozak CA, Chaganti RS, Posner JB, Old LJ, Cerebellar degeneration-related antigen: a highly conserved neuroectodermal marker mapped to chromosomes X in human and mouse. Proc Natl Acad Sci U S A87:3077-81 1990 |
| PubMed ID: 2326268 |
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| Rettig WJ, Grzeschik KH, Yenamandra AK, Garcia E, Old LJ, Definition of selectable cell surface markers for human chromosomes and chromosome segments in rodent-human hybrids. Somat Cell Mol Genet14:223-31 1988 |
| PubMed ID: 3163425 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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