GM07300
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/CHINESE HAMSTER SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Family Member
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23
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Human/Chinese hamster somatic cell hybrid retaining human chromosomes X, #6, #8, & #11
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for Xp22.32 (also Yp11.3), MIC2, and Xq28, DXS15. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp22.32, STS, Xq13.3, PGK1, Xq26, HPRT, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 5q33q35, PDGFRB. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 6pterp24, D6S7. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 8p22p12, NFL. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 8p12, D8S87, 8p21-p11.2, LHRH, 8p23-p22, DEF1, 8q11.22-q12, D8S260, 8q22.2, D8S270, 8q23-q24, D8S199, and 8q24, TG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 11p15.5, D11S12, and 11p13, CAT. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 19q13.3, LHB. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 21q22.1q22.2, D21S17. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. |
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| Remarks |
Line #1.11; human/HPRT-deficient Chinese hamster lung cell line; selected in medium containing azaserine; retains human chromosomes #6, #8, #11, & X in 60%, 20%, 40%, & 84% of cells respectively at passage 10 after recovery |
| Yealland G, Battaglia G, Bandmann O, Mortiboys H, Rescue of mitochondrial function in parkin-mutant Fibroblasts using drug loaded PMPC-PDPA polymersomes and tubular polymersomes Neuroscience letters: 2016 |
| PubMed ID: 27412236 |
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| Prawitt D, Enklaar T, Klemm G, Gartner B, Spangenberg C, Winterpacht A,
Higgins M, Pelletier J, Zabel B, Identification and characterization of MTR1, a novel gene with homology to
melastatin (MLSN1) and the trp gene family located in the BWS-WT2 critical
region on chromosome 11p15.5 and showing allele-specific expression. Curr Biol9(2):203-16 2000 |
| PubMed ID: 10607831 |
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| Anderson MJ, Viars CS, Czekay S, Cavenee WK, Arden KC, Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics47:187-99 1998 |
| PubMed ID: 9479491 |
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| Gabriel JM, Higgins MJ, Gebuhr TC, Shows TB, Saitoh S, Nicholls RD, A model system to study genomic imprinting of human genes. Proc Natl Acad Sci U S A95:14857-62 1998 |
| PubMed ID: 9843980 |
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| Arden KC, Viars CS, Fu K, Rozen R, Localization of short/branched chain acyl-CoA dehydrogenase (ACADSB) to human chromosome 10 Genomics25:743-5 1995 |
| PubMed ID: 7759115 |
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| Arden KC, Viars CS, Weiss S, Argentin S, Nemer M, Localization of the human B-type natriuretic peptide precursor (NPPB) gene to chromosome 1p36. Genomics26:385-9 1995 |
| PubMed ID: 7601467 |
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| Bermingham JR Jr, Arden KC, Naumova AK, Sapienza C, Viars CS, Fu XD, Khotz J, Manley JL, Rosenfeld MG, Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2). Genomics29:70-9 1995 |
| PubMed ID: 8530103 |
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| Kermouni A, Van Roost E, Arden KC, Vermeesch JR, Weiss S, Godelaine D, Flint J, Lurquin C, Szikora JP, Higgs DR, The IL-9 receptor gene (IL9R): genomic structure, chromosomal localization in the pseudoautosomal region of the long arm of the sex chromosomes, and identification of IL9R pseudogenes at 9qter, 10pter, 16pter, and 18pter Genomics29:371-82 1995 |
| PubMed ID: 8666384 |
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| Saito M, Helin K, Valentine MB, Griffith BB, Willman CL, Harlow E, Look AT, Amplification of the E2F1 transcription factor gene in the HEL erythroleukemia cell line. Genomics25:130-8 1995 |
| PubMed ID: 7774910 |
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| De Plaen E, Arden K, Traversari C, Gaforio JJ, Szikora JP, De Smet C, Brasseur F, van der Bruggen P, Lethé B, Lurquin C, Structure, chromosomal localization, and expression of 12 genes of the MAGE family Immunogenetics40:360-9 1994 |
| PubMed ID: 7927540 |
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| Hendershot LM, Valentine VA, Lee AS, Morris SW, Shapiro DN, Localization of the gene encoding human BiP/GRP78, the endoplasmic reticulum cognate of the HSP70 family, to chromosome 9q34. Genomics20:281-4 1994 |
| PubMed ID: 8020977 |
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| Huebner K, Kastury K, Druck T, Salcini AE, Lanfrancone L, Pelicci G, Lowenstein E, Li W, Park SH, Cannizzaro L, et al, Chromosome locations of genes encoding human signal transduction adapter proteins, Nck (NCK), Shc (SHC1), and Grb2 (GRB2). Genomics22:281-7 1994 |
| PubMed ID: 7806213 |
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| Kastury K, Druck T, Huebner K, Barletta C, Acampora D, Simeone A, Faiella A, Boncinelli E, Chromosome locations of human EMX and OTX genes. Genomics22:41-5 1994 |
| PubMed ID: 7959790 |
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| Seltmann M, Bohm M, Walter T, Schmitz G, Patterson D, Wieland I, Isolation of region specific single-copy probes from human chromosome 1q23-->1q25. Cytogenet Cell Genet67:46-51 1994 |
| PubMed ID: 8187551 |
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| Weiss J, Rubinfeld B, Polakis PG, McCormick F, Cavenee WK, Arden KC, The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps to chromosome 1p36.1-->p35. Cytogenet Cell Genet66:18-21 1994 |
| PubMed ID: 8275700 |
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| Dorig RE, Marcil A, Chopra A, Richardson CD, The human CD46 molecule is a receptor for measles virus (Edmonston strain). Cell75:295-305 1993 |
| PubMed ID: 8402913 |
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| Huebner K, Druck T, LaForgia S, Lasota J, Croce CM, Lanfrancone L, Donti E, Pengue G, La Mantia G, Pelicci PG, et al, Chromosomal localization of four human zinc finger cDNAs. Hum Genet91:217-22 1993 |
| PubMed ID: 8478004 |
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| Washington SS, Bowcock AM, Gerken S, Matsunami N, Lesh D, Osborne-Lawrence SL, Cowell J, Ledbetter DH, White RL, Chakravarti A, A somatic cell hybrid map of human chromosome 13. Genomics18:486-95 1993 |
| PubMed ID: 8307557 |
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| Morris SW, Nelson N, Valentine MB, Shapiro DN, Look AT, Kozlosky CJ, Beckmann MP, Cerretti DP, Assignment of the genes encoding human interleukin-8 receptor types 1 and 2 and an interleukin-8 receptor pseudogene to chromosome 2q35. Genomics14:685-91 1992 |
| PubMed ID: 1427896 |
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| Phaneuf D, Labelle Y, Bérubé D, Arden K, Cavenee W, Gagné R, Tanguay RM, Cloning and expression of the cDNA encoding human fumarylacetoacetate hydrolase, the enzyme deficient in hereditary tyrosinemia: assignment of the gene to chromosome 15 American journal of human genetics48:525-35 1991 |
| PubMed ID: 1998338 |
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| Arden KC, Boutin JM, Djiane J, Kelly PA, Cavenee WK, The receptors for prolactin and growth hormone are localized in the same region of human chromosome 5 Cytogenetics and cell genetics53:161-5 1990 |
| PubMed ID: 2369845 |
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| Scrable HJ, Johnson DK, Rinchik EM, Cavenee WK, Rhabdomyosarcoma-associated locus and MYOD1 are syntenic but separate loci on the short arm of human chromosome 11. Proc Natl Acad Sci U S A87:2182-6 1990 |
| PubMed ID: 2315312 |
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| Testa JR, Park M, Blair DG, Kalbakji A, Arden K, Vande Woude GF, Analysis by pulsed field gel electrophoresis reveals complex rearrangements in two MET alleles in a chemically-treated human cell line, MNNG-HOS Oncogene5:1565-71 1990 |
| PubMed ID: 2250912 |
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| Meeker TC, Grimaldi JC, O'Rourke R, Louie E, Juliusson G, Einhorn S, An additional breakpoint region in the BCL-1 locus associated with the t(11;14)(q13;q32) translocation of B-lymphocytic malignancy. Blood74:1801-6 1989 |
| PubMed ID: 2790203 |
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| Rettig WJ, Grzeschik KH, Yenamandra AK, Garcia E, Old LJ, Definition of selectable cell surface markers for human chromosomes and chromosome segments in rodent-human hybrids. Somat Cell Mol Genet14:223-31 1988 |
| PubMed ID: 3163425 |
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| Griffin CA, Emanuel BS, Hansen JR, Cavenee WK, Myers JC, Human collagen genes encoding basement membrane alpha 1 (IV) and alpha 2 (IV) chains map to the distal long arm of chromosome 13. Proc Natl Acad Sci U S A84:512-6 1987 |
| PubMed ID: 3025878 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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