GM07301
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/CHINESE HAMSTER SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Family Member
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12
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Reduced hybrid containing human chromosomes X and 12 in a Chinese hamster background
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Remarks
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for Xp22.3p22.2, DXS85, and Xq28, DXS15. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp22.32, STS, Xq13.3, PGK1, Xq26, HPRT, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 1p36 (also 3p21), DNF15S1/S2, and 1q32, REN. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 3p25, RAF1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 12p13.2, PRB1, and 12q14q24.1, D12S7. |
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| Remarks |
Line #16.2; human/HPRT-def Chinese hamster lung cell line; selected in medium containing azaserine; at passage 6, 96% of cells had human X & 92% of cells had human chromosome #12; original hybrid also contained the human Y chromosome |
| Anderson MJ, Viars CS, Czekay S, Cavenee WK, Arden KC, Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics47:187-99 1998 |
| PubMed ID: 9479491 |
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| Arden KC, Viars CS, Weiss S, Argentin S, Nemer M, Localization of the human B-type natriuretic peptide precursor (NPPB) gene to chromosome 1p36. Genomics26:385-9 1995 |
| PubMed ID: 7601467 |
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| Bermingham JR Jr, Arden KC, Naumova AK, Sapienza C, Viars CS, Fu XD, Khotz J, Manley JL, Rosenfeld MG, Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2). Genomics29:70-9 1995 |
| PubMed ID: 8530103 |
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| Saito M, Helin K, Valentine MB, Griffith BB, Willman CL, Harlow E, Look AT, Amplification of the E2F1 transcription factor gene in the HEL erythroleukemia cell line. Genomics25:130-8 1995 |
| PubMed ID: 7774910 |
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| De Plaen E, Arden K, Traversari C, Gaforio JJ, Szikora JP, De Smet C, Brasseur F, van der Bruggen P, Lethé B, Lurquin C, Structure, chromosomal localization, and expression of 12 genes of the MAGE family Immunogenetics40:360-9 1994 |
| PubMed ID: 7927540 |
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| Hendershot LM, Valentine VA, Lee AS, Morris SW, Shapiro DN, Localization of the gene encoding human BiP/GRP78, the endoplasmic reticulum cognate of the HSP70 family, to chromosome 9q34. Genomics20:281-4 1994 |
| PubMed ID: 8020977 |
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| Kastury K, Druck T, Huebner K, Barletta C, Acampora D, Simeone A, Faiella A, Boncinelli E, Chromosome locations of human EMX and OTX genes. Genomics22:41-5 1994 |
| PubMed ID: 7959790 |
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| Weiss J, Rubinfeld B, Polakis PG, McCormick F, Cavenee WK, Arden KC, The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps to chromosome 1p36.1-->p35. Cytogenet Cell Genet66:18-21 1994 |
| PubMed ID: 8275700 |
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| Washington SS, Bowcock AM, Gerken S, Matsunami N, Lesh D, Osborne-Lawrence SL, Cowell J, Ledbetter DH, White RL, Chakravarti A, A somatic cell hybrid map of human chromosome 13. Genomics18:486-95 1993 |
| PubMed ID: 8307557 |
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| Board PG, Coggan M, Baker RT, Vuust J, Webb GC, Localization of the human UBC polyubiquitin gene to chromosome band 12q24.3. Genomics12:639-42 1992 |
| PubMed ID: 1315303 |
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| Board PG, Coggan M, Woodcock DM, The human Pi class glutathione transferase sequence at 12q13-q14 is a reverse-transcribed pseudogene. Genomics14:470-3 1992 |
| PubMed ID: 1427860 |
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| Morris SW, Nelson N, Valentine MB, Shapiro DN, Look AT, Kozlosky CJ, Beckmann MP, Cerretti DP, Assignment of the genes encoding human interleukin-8 receptor types 1 and 2 and an interleukin-8 receptor pseudogene to chromosome 2q35. Genomics14:685-91 1992 |
| PubMed ID: 1427896 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 X 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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