Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
| Class |
Disorders of the Nervous System |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
|
|
Race
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White
|
|
Family Member
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1
|
|
Relation to Proband
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proband
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|
Confirmation
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Clinical summary/Case history
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|
Species
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Homo sapiens
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Common Name
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Human
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Remarks
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|
| PDL at Freeze |
7.03 |
| Passage Frozen |
2 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
See GM7544 Lymphoid |
| de Andrade KC, Pinto EM, Zhao T, Zeigler LP, Kim J, Giri N, Haley JS, McReynolds LJ, Florez-Vargas O, Phillips AH, Kriwacki RW, Akinniyi SA, Cohen SB, Emerson MR, Smelser DT, Urban GM, Fridman C, Zambetti GP, Bryan TM, Carey DJ, Garcia CK, Stewart DR, Savage SA, TERT c3150 G > C (pK1050N): a founder Ashkenazi Jewish variant associated with telomere biology disorders NPJ genomic medicine10:46 2025 |
| PubMed ID: 40456748 |
| |
| Le T, Ferling I, Qiu L, Nabaile C, Assunção L, Roskelley CD, Grinstein S, Freeman SA, Redistribution of the glycocalyx exposes phagocytic determinants on apoptotic cells Developmental cell59:853-868.e7 2024 |
| PubMed ID: 38359833 |
| |
| Wilson E, Knudson W, Newell-Litwa K, Hyaluronan regulates synapse formation and function in developing neural networks Scientific reports10:16459 2020 |
| PubMed ID: 33020512 |
| |
| Porterfield V, Khan SS, Foff EP, Koseoglu MM, Blanco IK, Jayaraman S, Lien E, McConnell MJ, Bloom GS, Lazo JS, Sharlow ER, A three-dimensional dementia model reveals spontaneous cell cycle re-entry and a senescence-associated secretory phenotype Neurobiology of aging90:125-134 2019 |
| PubMed ID: 32184029 |
| |
| Wilson E, Rudisill T, Kirk B, Johnson C, Kemper P, Newell-Litwa K, Cytoskeletal regulation of synaptogenesis in a model of human fetal brain development Journal of neuroscience research90:125-134 2019 |
| PubMed ID: 32713041 |
| Passage Frozen |
2 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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