Description:
TRANSLOCATED CHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Cell Type
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Fibroblast
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|
Transformant
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Untransformed
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|
Race
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White
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|
Ethnicity
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ITALIAN
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|
Family Member
|
2
|
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Relation to Proband
|
proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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46,XY,t(4;14)(q25;q32)[19]
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Species
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Homo sapiens
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Common Name
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Human
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|
Remarks
|
|
| PDL at Freeze |
4.32 |
| Passage Frozen |
5 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Cytogenetics |
Chromosome 14: TRANSLOCATION Breakpoint 14q32 t(4;14)14q32 |
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Chromosome 4: TRANSLOCATION Breakpoint 4q25 t(4;14)4q25 |
| Remarks |
Daughter with same balanced translocation has had 3 spontaneous abortions |
| Passage Frozen |
5 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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