GM10157
Somatic cell hybrid
Description:
CHROMOSOME 21 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
ADDITIONAL DELETION/DERIVATIVE Y CHROMOSOME SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Chinese hamster hybrid retaining a human der(Y;21)t(Ypter>Yp10::21q10> 21qter) in 96% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line 72532X; norm hum lymphocytes fused with CHO cells (Ade-G mutant) using UVinact Sendai virus; selected in F12 medium minus hypoxanthine & 5% macroserum (dialyzed); retains hu der(Y;21)t(Ypter> Yp10::21q10>21qter) in 96% of cells |
| Chastain-Moore AM, Roberts T, Trott DA, Newbold RF, Ornelles DA, An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression. J Virol77(14):8087-98 2003 |
| PubMed ID: 12829847 |
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| Van Keuren ML, Watkins PC, Drabkin HA, Jabs EW, Gusella JF, Patterson D, Regional localization of DNA sequences on chromosome 21 using somatic cell hybrids. Am J Hum Genet38:793-804 1986 |
| PubMed ID: 3014865 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 deficient Medium with 2mM L-glutamine or equivalent |
| Serum |
5% dialyzed fetal bovine serum |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 50mg/ml stock |
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