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GM10324 Somatic cell hybrid

Description:

ADDITIONAL HUMAN/MOUSE SOMATIC CELL HYBRIDS

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Hybrids
Cell Type Somatic cell hybrid
Transformant Untransformed
Family Member 23
Confirmation Karyotypic analysis and Southern blot hybridization
ISCN Human/Mouse somatic cell hybrid retaining the human X chromosome in 90% of cells
Remarks Line AHA 11a; produced by fusing human fibroblasts, GM00144, 46,XX,-6,+der(6) t(6;21), with the HPRT- and APRT-deficient mouse cell line A9; selected in HAT medium; 90% of cells retain the human X chromosome

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 
GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for Xp22.2, DXS16, and Xq28, DXS15. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp11.3, DXS1003, Xp22.32, STS, Xq12, DXS453, Xq13.3, PGK1, Xq21.3, DXS3, Xq26, HPRT, and Xq28, G6PD.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 3q26.2qter, TFRC.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 5pterp15.3, D5S10.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 12p13, TPI1.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 16pterp13, D16S36, and 16q13q22.1, MT2.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 18q23, MBP.
 

Phenotypic Data

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Remarks Line AHA 11a; produced by fusing human fibroblasts, GM00144, 46,XX,-6,+der(6) t(6;21), with the HPRT- and APRT-deficient mouse cell line A9; selected in HAT medium; 90% of cells retain the human X chromosome

Publications

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Coskun AK, van Maanen M, Nguyen V, Sutton RE, Human chromosome 2 carries a gene required for production of infectious human immunodeficiency virus type 1 Journal of virology80:3406-15 2006
PubMed ID: 16537608
 
Kubota T, Nonoyama S, Tonoki H, Masuno M, Imaizumi K, Kojima M, Wakui K, Shimadzu M, Fukushima Y, A new assay for the analysis of X-chromosome inactivation based on methylation-specific PCR. Hum Genet104(1):49-55 1999
PubMed ID: 10071192
 
Swanson DA, Freund CL, Ploder L, McInnes RR, Valle D, A ubiquitin C-terminal hydrolase gene on the proximal short arm of the X chromosome: implications for X-linked retinal disorders Human molecular genetics5:533-8 1996
PubMed ID: 8845848
 
Bloch KD, Wolfram JR, Brown DM, Roberts JD Jr, Zapol DG, Lepore JJ, Filippov G, Thomas JE, Jacob HJ, Bloch DB, Three members of the nitric oxide synthase II gene family (NOS2A, NOS2B, and NOS2C) colocalize to human chromosome 17. Genomics27:526-30 1995
PubMed ID: 7558036
 
Saito M, Helin K, Valentine MB, Griffith BB, Willman CL, Harlow E, Look AT, Amplification of the E2F1 transcription factor gene in the HEL erythroleukemia cell line. Genomics25:130-8 1995
PubMed ID: 7774910
 
Fukushima A, Okubo K, Sugino H, Hori N, Matoba R, Niiyama T, Murakawa K, Yoshii J, Yokoyama M, Matsubara K, Chromosomal assignment of HepG2 3'-directed partial cDNA sequences by Southern blot hybridization using monochromosomal hybrid cell panels. Genomics22:127-36 1994
PubMed ID: 7959758
 
Hendershot LM, Valentine VA, Lee AS, Morris SW, Shapiro DN, Localization of the gene encoding human BiP/GRP78, the endoplasmic reticulum cognate of the HSP70 family, to chromosome 9q34. Genomics20:281-4 1994
PubMed ID: 8020977
 
Drwinga HL, Toji LH, Kim CH, Greene AE, Mulivor RA, NIGMS human/rodent somatic cell hybrid mapping panels 1 and 2. Genomics16:311-4 1993
PubMed ID: 8314568
 
Howe JR, Lairmore TC, Veile R, Dou S, Wells SA Jr, Donis-Keller H, Development of a sequence-tagged site for the centromere of chromosome 10: its use in cytogenetic and physical mapping. Hum Genet91:199-204 1993
PubMed ID: 8478002
 
Morris SW, Nelson N, Valentine MB, Shapiro DN, Look AT, Kozlosky CJ, Beckmann MP, Cerretti DP, Assignment of the genes encoding human interleukin-8 receptor types 1 and 2 and an interleukin-8 receptor pseudogene to chromosome 2q35. Genomics14:685-91 1992
PubMed ID: 1427896
 
Dorman BP, Shimizu N, Ruddle FH, Genetic analysis of the human cell surface: antigenic marker for the human X chromosome in human-mouse hybrids. Proc Natl Acad Sci U S A75:2363-7 1978
PubMed ID: 353811

External Links

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dbSNP dbSNP ID: 11358

Culture Protocols

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Split Ratio 1:3
Temperature 37 C
Percent CO2 8%
Medium Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent
Serum 10% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA
Supplement Aminopterin 4 x 10-7M
Supplement Hypoxanthine 1 x 10-4M
Supplement Thymidine 1.6 x 10-5M
Pricing
International/Commercial/For-profit:
$373.00USD
U.S. Academic/Non-profit/Government:
$216.00USD
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