GM10500
Somatic cell hybrid
Description:
CHROMOSOME 15 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Human/mouse hybrid retaining a human der(15)t(15;17) chromosome in 100% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for Xp22.32 and Yp11.3, MIC2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 10q24qter, PLAU. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 14q32.1q32.3, D14S13. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 15q25qter, IGF1R. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 15q11-q12, D15S11, 15q21-q22.2, B2M, and 15q11.2-q12, GABRB3, and gave a negative result with a primer for 15q23, D15S1485A. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17p13.1, MYH2, and gave a positive result with a probe for 17q21.3q23, MPO. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 17p13.1, TP53, and 17q21.3-q23, MPO1, and gave positive results with primers for 17q, GAS, and 17q25-qter, D17S784. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. |
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| Remarks |
Line SP3-10; produced by fusing human fibroblasts, 46,XX,t(15;17)(q22;q11.2), with LMTK- mouse cells; selected in HAT medium with ouabain; retains the der(15) t(15;17) as the only human chromosome in 100% of cells |
| Plummer SJ, Simmons JA, Adams L, Casey G, Mapping of 228 ESTs and 26 genes into an integrated physical and genetic map of human chromosome 17. Genomics45:140-6 1997 |
| PubMed ID: 9339370 |
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| Kusuda J, Hidari N, Hirai M, Hashimoto K, Sequence analysis of the cDNA for the human casein kinase I delta (CSNK1D) gene and its chromosomal localization. Genomics32:140-3 1996 |
| PubMed ID: 8786104 |
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| Argeson AC, Druck T, Veronese ML, Knopf JL, Buchberg AM, Huebner K, Siracusa LD, Phospholipase C gamma-2 (Plcg2) and phospholipase C gamma-1 (Plcg1) map to distinct regions in the human and mouse genomes. Genomics25:29-35 1995 |
| PubMed ID: 7774933 |
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| Carr IM, Markham AF, Molecular genetic analysis of the human sorbitol dehydrogenase gene Mammalian genome : official journal of the International Mammalian Genome Society6:645-52 1995 |
| PubMed ID: 8535074 |
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| Kusuda J, Tanuma R, Gotoh E, Toyoda A, Hashimoto K, Assignment of a human autoimmune antigen, p80-coilin gene to chromosome 17q21-q23 and of its possible pseudogene to chromosome 14. Hum Genet95:233-4 1995 |
| PubMed ID: 7860074 |
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| Polymeropoulos MH, Torres R, Yanovski JA, Chandrasekharappa SC, Ledbetter DH, The human corticotropin-releasing factor receptor (CRHR) gene maps to chromosome 17q12-q22. Genomics28:123-4 1995 |
| PubMed ID: 7590738 |
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| Chang DY, Nelson B, Bilyeu T, Hsu K, Darlington GJ, Maraia RJ, A human Alu RNA-binding protein whose expression is associated with accumulation of small cytoplasmic Alu RNA. Mol Cell Biol14:3949-59 1994 |
| PubMed ID: 8196634 |
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| Driscoll CT, Darlington GJ, Maraia RJ, The conserved 7SK snRNA gene localizes to human chromosome 6 by homolog
exclusion probing of somatic cell hybrid RNA. Nucleic Acids Res22(5):722-5 1994 |
| PubMed ID: 8139910 |
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| Fougerousse F, Broux O, Richard I, Allamand V, de Souza AP, Bourg N, Brenguier L, Devaud C, Pasturaud P, Roudaut C, Mapping of a chromosome 15 region involved in limb girdle muscular dystrophy Human molecular genetics3:285-93 1994 |
| PubMed ID: 8004096 |
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| Richard I, Broux O, Chiannilkulchai N, Fougerousse F, Allamand V, Bourg N, Brenguier L, Devaud C, Pasturaud P, Roudaut C, Regional localization of human chromosome 15 loci Genomics23:619-27 1994 |
| PubMed ID: 7851890 |
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| Anderson LA, Friedman L, Osborne-Lawrence S, Lynch E, Weissenbach J, Bowcock A, King MC, High-density genetic map of the BRCA1 region of chromosome 17q12-q21. Genomics17:618-23 1993 |
| PubMed ID: 8244378 |
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| van Tuinen P, Rich DC, Summers KM, Ledbetter DH, Regional mapping panel for human chromosome 17: application to neurofibromatosis type 1. Genomics1:374-81 1987 |
| PubMed ID: 3130306 |
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| Sheer D, Sheppard DM, le Beau M, Rowley JD, San Roman C, Solomon E, Localization of the oncogene c-erbA1 immediately proximal to the acute promyelocytic leukaemia breakpoint on chromosome 17. Ann Hum Genet49 ( Pt 3):167-71 1985 |
| PubMed ID: 3865620 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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