GM10502
Somatic cell hybrid
Description:
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Human/mouse hybrid retaining human chromosomes #5, 6, 7, & der(19)t(17;19)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 15q25qter, IGF1R. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 17p13.1, MYH2, and 17q21.3q23, MPO, and gave a positive result with a probe for 17q25, P4HB. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 17q25-qter, D17S784, and gave negative results with primers for 17p13.1, TP53, 17q21.3-q23, MPO1, and 17q, GAS. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 19p13.1, D19S11, and 19q13.3, LHB. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 19p13.2, LDLR, and 19q13.2, APOC2. |
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| Remarks |
Line MH-41; produced by peg fusion of human fibros, GM00271, 46,XX,t(17;19) (q23;p13), with LMTK- mouse cells; selected in HAT medium containing ouabain; retains human chromosomes der(19) (100% of cells), #5 (36%), 6 (56%) & 7 (56%) |
| Moran JL, Johnston SH, Rauskolb C, Bhalerao J, Bowcock AM, Vogt TF, Genomic structure, mapping, and expression analysis of the mammalian Lunatic,
Manic, and Radical fringe genes. Mamm Genome10(6):535-41 1999 |
| PubMed ID: 10341080 |
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| Pelletier GJ, Brody SL, Liapis H, White RA, Hackett BP, A human forkhead/winged-helix transcription factor expressed in developing pulmonary and renal epithelium. Am J Physiol274:L351-9 1998 |
| PubMed ID: 9530170 |
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| Plummer SJ, Simmons JA, Adams L, Casey G, Mapping of 228 ESTs and 26 genes into an integrated physical and genetic map of human chromosome 17. Genomics45:140-6 1997 |
| PubMed ID: 9339370 |
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| Ishizuka T, Ahmad I, Kita K, Sonoda T, Ishijima S, Sawa K, Suzuki N, Tatibana M, The human phosphoribosylpyrophosphate synthetase-associated protein 39 gene (PRPSAP1) is located in the chromosome region 17q24-q25. Genomics33:332-4 1996 |
| PubMed ID: 8660991 |
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| Kusuda J, Hidari N, Hirai M, Hashimoto K, Sequence analysis of the cDNA for the human casein kinase I delta (CSNK1D) gene and its chromosomal localization. Genomics32:140-3 1996 |
| PubMed ID: 8786104 |
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| Brody LC, Abel KJ, Castilla LH, Couch FJ, McKinley DR, Yin G, Ho PP, Merajver S, Chandrasekharappa SC, Xu J, et al, Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17. Genomics25:238-47 1995 |
| PubMed ID: 7774924 |
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| Kusuda J, Tanuma R, Gotoh E, Toyoda A, Hashimoto K, Assignment of a human autoimmune antigen, p80-coilin gene to chromosome 17q21-q23 and of its possible pseudogene to chromosome 14. Hum Genet95:233-4 1995 |
| PubMed ID: 7860074 |
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| Polymeropoulos MH, Torres R, Yanovski JA, Chandrasekharappa SC, Ledbetter DH, The human corticotropin-releasing factor receptor (CRHR) gene maps to chromosome 17q12-q22. Genomics28:123-4 1995 |
| PubMed ID: 7590738 |
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| O'Connell P, Albertsen H, Matsunami N, Taylor T, Hundley JE, Johnson-Pais TL, Reus B, Lawrence E, Ballard L, White R, et al, A radiation hybrid map of the BRCA1 region. Am J Hum Genet54:526-34 1994 |
| PubMed ID: 8116622 |
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| Anderson LA, Friedman L, Osborne-Lawrence S, Lynch E, Weissenbach J, Bowcock A, King MC, High-density genetic map of the BRCA1 region of chromosome 17q12-q21. Genomics17:618-23 1993 |
| PubMed ID: 8244378 |
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| Virtaneva KI, Angelisova P, Baumruker T, Horejsi V, Nevanlinna H, Schroder J, The genes for CD37, CD53, and R2, all members of a novel gene family, are located on different chromosomes. Immunogenetics37:461-5 1993 |
| PubMed ID: 8436422 |
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| van Tuinen P, Rich DC, Summers KM, Ledbetter DH, Regional mapping panel for human chromosome 17: application to neurofibromatosis type 1. Genomics1:374-81 1987 |
| PubMed ID: 3130306 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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