GM10890
Somatic cell hybrid
Description:
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Mouse/human somatic cell hybrid retaining a human der(Y) chromosome
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for Yq12, DYZ1, and gave a positive result with a probe for Yp11.3 and Xp22.32, DXYS20. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17p13.1, MYH2, and gave a positive result with a probe for 17q21.3q23, MPO. |
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| Remarks |
Line Y17-41; produced by fusing GM06326, 46,X,t(Y;17)(q11;q12), with the TK-deficient mouse cell line Cl1D; selected in HAT medium; retains the human der(Y) in 100% of cells & no other intact human chromosomes |
| Penin J, Dufour S, Faure V, Fritah S, Seigneurin-Berny D, Col E, Verdel A, Vourc'h C, Chromosome Y pericentric heterochromatin is a primary target of HSF1 in male cells Chromosoma130:53-60 2020 |
| PubMed ID: 33547955 |
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| Plummer SJ, Simmons JA, Adams L, Casey G, Mapping of 228 ESTs and 26 genes into an integrated physical and genetic map of human chromosome 17. Genomics45:140-6 1997 |
| PubMed ID: 9339370 |
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| Verdier AS, Mattei MG, Lovec H, Hartung H, Goldfarb M, Birnbaum D, Coulier F, Chromosomal mapping of two novel human FGF genes, FGF11 and FGF12. Genomics40:151-4 1997 |
| PubMed ID: 9070933 |
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| Bukvic N, Susca F, Gentile M, Tangari E, Ianniruberto A, Guanti G, An unusual dicentric Y chromosome with a functional centromere with no detectable alpha-satellite. Hum Genet97:453-6 1996 |
| PubMed ID: 8834241 |
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| Kusuda J, Hidari N, Hirai M, Hashimoto K, Sequence analysis of the cDNA for the human casein kinase I delta (CSNK1D) gene and its chromosomal localization. Genomics32:140-3 1996 |
| PubMed ID: 8786104 |
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| Kusuda J, Tanuma R, Gotoh E, Toyoda A, Hashimoto K, Assignment of a human autoimmune antigen, p80-coilin gene to chromosome 17q21-q23 and of its possible pseudogene to chromosome 14. Hum Genet95:233-4 1995 |
| PubMed ID: 7860074 |
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| Polymeropoulos MH, Torres R, Yanovski JA, Chandrasekharappa SC, Ledbetter DH, The human corticotropin-releasing factor receptor (CRHR) gene maps to chromosome 17q12-q22. Genomics28:123-4 1995 |
| PubMed ID: 7590738 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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