GM11219
Fibroblast from Whole embryo, Embryo
Description:
TRANSLOCATED CHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Biopsy Source
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Embryo
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Cell Type
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Fibroblast
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Tissue Type
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Whole embryo
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Whole embryo, Embryo
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XX,t(6;11)(6qter>6p23::11q21>11qter;11pter>11q21::6p23>6pter)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
5 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Cytogenetics |
Chromosome 11: TRANSLOCATION Breakpoint 11q21 t(6;11)11q21 |
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Chromosome 6: TRANSLOCATION Breakpoint 6p23 t(6;11)6p23 |
| Remarks |
Product of conception fibroblast culture presumed to be maternal cell contamination; mother is carrier of t(6;11) and phenotypically normal |
| Passage Frozen |
5 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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