GM11436
Somatic cell hybrid
Description:
CHROMOSOME 5 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 4 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Human/Chinese hamster somatic cell hybrid retaining a human der(5)t(4;5) (p15.2;p14.3) in 100% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 4p16.2p15.1, D4S20, and a negative result with a probe for 4q21q23, ADH3. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 4p15.3, QDPR, 4p16.3, PDEB.2, and 4p16.3, D4S126, and gave negative results with primers for 4q11-q13, AFP, 4q25, EGF, 4q28, FGA, 4q31-q33, D4S1626, and 4q35, F11. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 5pterp15.3, D5S10, and gave a positive result with a probe for 5q33q35, PDGFRB. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 5p15.3-p15.1, D5S208, and gave positive results with primers for 5p14-p12, C9, 5q11.2-q13.1, D5S351, 5q13.3-q14, HMGCR, 5q32, D5S372, and 5q34-q35, GABRA1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 12q14q24.1, D12S7, and 12p13, TPI1. |
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| Remarks |
Line HHW661; human/Chinese hamster somatic cell hybrid retaining a human der(5)t(4;5)(p15.2;p14.3) in 100% of cells; grown in culture medium containing 0.2mM proline at 39C |
| Soltysik-Espanola M, Rogers RA, Jiang S, Kim TA, Gaedigk R, White RA, Avraham H, Avraham S, Characterization of Mayven, a novel actin-binding protein predominantly expressed in brain. Mol Biol Cell10:2361-75 1999 |
| PubMed ID: 10397770 |
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| Inazawa J, Ariyama T, Abe T, Druck T, Ohta M, Huebner K, Yanagisawa J, Reed JC, Sato T, PTPN13, a fas-associated protein tyrosine phosphatase, is located on the long arm of chromosome 4 at band q21.3. Genomics31:240-2 1996 |
| PubMed ID: 8824809 |
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| Wieland I, Bohm M, Arden KC, Ammermuller T, Bogatz S, Viars CS, Rajewsky MF, Allelic deletion mapping on chromosome 5 in human carcinomas. Oncogene12:97-102 1996 |
| PubMed ID: 8552404 |
| Split Ratio |
1:5 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
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