GM11443
Somatic cell hybrid
Description:
CHROMOSOME 5 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Human/Chinese hamster hybrid retaining a human del(5) in 96% of cells, #1 (76%), & #19 (96%)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 1q32, REN. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 4p16.2p15.1, D4S20, and 4q21q23, ADH3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 5pterp15.3, D5S10, and 5q33q35, PDGFRB. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 5p14-p12, C9, 5p15.3-p15.1, D5S208, 5q11.2q13.1, D5S351, 5q13.3-q14, HMGCR, 5q32, D5S372, and 5q34-q35, GABRA1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 9q34.1, ABL. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 12q14q24.1, D12S7, and 12p13, TPI1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 16q13q22.1, MT2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 18q12.3, D18S6. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 19q13.3, LHB. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 19q13.2, APOC2. |
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| Remarks |
Line HHW1113; human/Chinese hamster somatic cell hybrid; selected in medium containing 0.2mM proline at 39C; retains human del(5)(q15q21.3) in 96% of cells, #1 in 76% of cells, & #19 in 96% of cells |
| Cerretti DP, Copeland NG, Gilbert DJ, Jenkins NA, Kuefer MU, Valentine V, Shapiro DN, Cui X, Morris SW, The gene encoding LERK-7 (EPLG7, Epl7), a ligand for the Eph-related receptor tyrosine kinases, maps to human chromosome 5 at band q21 and to mouse chromosome 17. Genomics35:376-9 1996 |
| PubMed ID: 8661153 |
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| Hobson GM, Krahe R, Garcia E, Siciliano MJ, Funanage VL, Regional chromosomal assignments for four members of the MADS domain transcription enhancer factor 2 (MEF2) gene family to human chromosomes 15q26, 19p12, 5q14, and 1q12-q23. Genomics29:704-11 1995 |
| PubMed ID: 8575763 |
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| McPherson JD, Morton RA, Ewing CM, Wasmuth JJ, Overhauser J, Nagafuchi A, Tsukita S, Isaacs WB, Assignment of the human alpha-catenin gene (CTNNA1) to chromosome 5q21- q22. Genomics19:188-90 1994 |
| PubMed ID: 8188230 |
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| Nagarajan L, Zavadil J, Claxton D, Lu X, Fairman J, Warrington JA, Wasmuth JJ, Chinault AC, Sever CE, Slovak ML, et al, Consistent loss of the D5S89 locus mapping telomeric to the interleukin gene cluster and centromeric to EGR-1 in patients with 5q- chromosome. Blood83:199-208 1994 |
| PubMed ID: 8274735 |
| Split Ratio |
1:5 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
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