GM11444
Somatic cell hybrid
Description:
CHROMOSOME 5 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 2 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/hamster hybrid retaining human der(2)t(2;5) in 92% of cells & #4, 6, 10, 12, 16, 18, & 21
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 2p25, D2S1, and gave a positive result with a probe for 2q37, D2S3. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 2p13, TGFA, and 2p23, POMC, and gave positive results with primers for 2q13, IL1A, and 2q37, ALPP. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 3p25, RAF1, and 3q26.2qter, TFRC. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 5pterp15.3, D5S10, and gave a positive result with a probe for 5q33q35, PDGFRB. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 5p14-p12, C9, 5p15.3-p15.1, D5S208, 5q11.2q13.1, D5S351, and 5q13.3-q14, HMGCR, and gave positive results with primers for 5q32, D5S372, and 5q34-q35, GABRA1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 18q12.3, D18S6. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 21q11, D21S26, and 21q22.1q22.2, D21S17. |
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| Remarks |
Line HHW1124; human/Chinese hamster somatic cell hybrid; selected in medium containing 0.2mM proline at 39C; retains human der(2)t(2:5)(p11.2;q22) in 92% of cells & other human chromosomes |
| Jones MH, Zhang Y, Tirosvoutis KN, Davey PM, Webster AR, Walsh D, Spurr NK, Affara NA, Chromosomal assignment of 311 sequences transcribed in human adult testis. Genomics40:155-67 1997 |
| PubMed ID: 9070934 |
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| Liang Y, Chen H, Asher JH Jr, Chang CC, Friedman TB, Human inner ear OCP2 cDNA maps to 5q22-5q35.2 with related sequences on chromosomes 4p16.2-4p14, 5p13-5q22, 7pter-q22, 10 and 12p13-12qter. Gene184:163-7 1997 |
| PubMed ID: 9031623 |
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| Wedemeyer N, Lengeling A, Ronsiek M, Korthaus D, Baer K, Wuttke M, Jockusch H, YAC contigs of the Rab1 and wobbler (wr) spinal muscular atrophy gene region on proximal mouse chromosome 11 and of the homologous region on human chromosome 2p. Genomics32:447-54 1996 |
| PubMed ID: 8838809 |
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| Hobson GM, Krahe R, Garcia E, Siciliano MJ, Funanage VL, Regional chromosomal assignments for four members of the MADS domain transcription enhancer factor 2 (MEF2) gene family to human chromosomes 15q26, 19p12, 5q14, and 1q12-q23. Genomics29:704-11 1995 |
| PubMed ID: 8575763 |
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| McPherson JD, Morton RA, Ewing CM, Wasmuth JJ, Overhauser J, Nagafuchi A, Tsukita S, Isaacs WB, Assignment of the human alpha-catenin gene (CTNNA1) to chromosome 5q21- q22. Genomics19:188-90 1994 |
| PubMed ID: 8188230 |
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| Nagarajan L, Zavadil J, Claxton D, Lu X, Fairman J, Warrington JA, Wasmuth JJ, Chinault AC, Sever CE, Slovak ML, et al, Consistent loss of the D5S89 locus mapping telomeric to the interleukin gene cluster and centromeric to EGR-1 in patients with 5q- chromosome. Blood83:199-208 1994 |
| PubMed ID: 8274735 |
| Split Ratio |
1:5 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
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