GM11446
Somatic cell hybrid
Description:
CHROMOSOME 7 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 5 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Chinese hamster/human hybrid retaining human der(5)t(5;7) in 100% of cells & X in 4% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for Xp22.32 and Yp11.3, MIC2. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp22.32, STS, Xq13.3, PGK1, Xq26, HPRT, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 4p16.2p15.1, D4S20, and 4q21q23, ADH3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 5pterp15.3, D5S10, and 5q33q35, PDGFRB. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 7pterp15, ASSP11, and gave a positive result with a probe for 7q31.1q31.2, D7S18. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 12p13.2, PRB1, and 12q14q24.1, D12S7. |
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| Remarks |
Line HHW1107; human/Chinese hamster somatic cell hybrid retaining a human der(5)t(5;7)(5pter>5q35.2::7q22>7qter) in 100% of cells & X in 4%; grown in medium containing 0.2mM proline at 39C |
| Liang Y, Chen H, Asher JH Jr, Chang CC, Friedman TB, Human inner ear OCP2 cDNA maps to 5q22-5q35.2 with related sequences on chromosomes 4p16.2-4p14, 5p13-5q22, 7pter-q22, 10 and 12p13-12qter. Gene184:163-7 1997 |
| PubMed ID: 9031623 |
| Split Ratio |
1:25 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
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