GM11534
Tumor-derived cell line from Neuroepithelioma
Description:
TRANSLOCATED CHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Biopsy Source
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Neuroepithelioma
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Cell Type
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Tumor-derived cell line
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Tissue Type
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Tumor
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Transformant
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Untransformed
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Sample Source
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Tumor-derived cell line from Neuroepithelioma
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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56,XY,t(11;22)(q24;q12),+5,+7,+8,+15, +18,+20,+21,+der(22)t(11;22)(q24;q12) +mars
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Cytogenetics |
Chromosome 22: TRANSLOCATION Breakpoint 22q12 t(11;22)22q12 |
| Remarks |
Peripheral neuroepithelioma derived cell line N1000; EM exam of tumor showed classic neurosecretory granules & neuritic processes; line tested pos for the presence of catecholamine metabolites & neuron specific enolase; pass 1 at CCR |
| Israel, Peripheral neuroepithelioma: Genetic analysis of tumor derived cell lines. Adv Neuroblastoma Res: 1985 |
| PubMed ID: |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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