GM11685
Somatic cell hybrid
Description:
CHROMOSOME 22 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 17 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/mouse hybrid retaining human 17qter>17q11.2::22q11.2>cen translocated to a mouse chromo & #3
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 17p13.1, TP53, and gave positive results with primers for 17q21.3-q23, MPO1, and 17q25-qter, D17S784. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 22q11.2, IGLC2, 22q12.1-q13.1, D22S300, 22q13.31, KI-108, and 22q13.31-qter, DIA1. |
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| Remarks |
Line NF13-CL.D3; neurofibromatosis pt lymphoblasts, t(17;22)(q11.2;q11.2), fused with TK-def Cl1D mouse cells; grown in HAT medium; retains hum 17qter> 17q11.2::22q11.2>22cen translocated to a mouse chromo (93% cells) & hum chromo #3 |
| Kurahashi H, Inagaki H, Hosoba E, Kato T, Ohye T, Kogo H, Emanuel BS, Molecular cloning of a translocation breakpoint hotspot in 22q11 Genome research17:461-9 2007 |
| PubMed ID: 17267815 |
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| Budarf ML, Eckman B, Michaud D, McDonald T, Gavigan S, Buetow KH, Tatsumura Y, Liu Z, Hilliard C, Driscoll D, Goldmuntz E, Meese E, Zwarthoff EC, Williams S, McDermid H, Dumanski JP, Biegel J, Bell CJ, Emanuel BS, Regional localization of over 300 loci on human chromosome 22 using a somatic cell hybrid mapping panel. Genomics35:275-88 1996 |
| PubMed ID: 8661140 |
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| Bell CJ, Budarf ML, Nieuwenhuijsen BW, Barnoski BL, Buetow KH, Campbell K, Colbert AM, Collins J, Daly M, Desjardins PR, et al, Integration of physical, breakpoint and genetic maps of chromosome 22. Localization of 587 yeast artificial chromosomes with 238 mapped markers. Hum Mol Genet4:59-69 1995 |
| PubMed ID: 7711735 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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