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GM11750 Somatic cell hybrid

Description:

CHROMOSOME 3 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Hybrids
Cell Type Somatic cell hybrid
Transformant Untransformed
Confirmation Clinical summary/Case history
ISCN Mouse/human hybrid retaining human der(X)t(X;3)
Remarks Line R1-1; produced by the fusion of human lymphoblasts, der(X)t(X;3)(3pter> 3p13::Xp2?>Xqter), with mouse RAG cells; grown in HAT medium; retains human 3pter>p13 in 92% of cells

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 
GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp11.3, DXS1003, Xq12, DXS453, Xq13.3, PGK1, Xq21.3, DXS3, Xq26, HPRT, and Xq28, G6PD, and gave a negative result with a primer for Xp22.32, STS.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 1p13, NGFB.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 3p14.1-p12, D3S1566, 3p22-p21.33, GLB1, and 3p25-p24.2, D3S1304, and gave negative results with primers for 3q13.1-q13.2, D3S1310, 3q22-q24, D3S1764, 3q26.2-q27, GLUT2, and 3q28q29, D3S1311.
 

Phenotypic Data

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Remarks Line R1-1; produced by the fusion of human lymphoblasts, der(X)t(X;3)(3pter> 3p13::Xp2?>Xqter), with mouse RAG cells; grown in HAT medium; retains human 3pter>p13 in 92% of cells

Publications

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Maassen VanDenBrink A, Vergouwe MN, Ophoff RA, Naylor SL, Dauwerse HG, Saxena PR, Ferrari MD, Frants RR, Chromosomal localization of the 5-HT1F receptor gene: no evidence for involvement in response to sumatriptan in migraine patients. Am J Med Genet77(5):415-20 1998
PubMed ID: 9632173
 
Ayyanathan K, Naylor SL, Kunapuli SP, Structural characterization and fine chromosomal mapping of the human P2Y1 purinergic receptor gene (P2RY1). Somat Cell Mol Genet22:419-24 1996
PubMed ID: 9039850
 
Bailey A, Norris AL, Leek JP, Clissold PM, Carr IM, Ogilvie DJ, Morrison JF, Meredith DM, Markham AF, Yeast artificial chromosome cloning of the beta-catenin locus on human chromosome 3p21-22. Chromosome Res3:201-3 1995
PubMed ID: 7780664
 
Naylor SL, Buys CH, Carritt B, Report and abstracts of the Fourth International Workshop on Human Chromosome 3 Mapping Cytogenetics and cell genetics65:2-50 1994
PubMed ID: 8104766
 
Drabkin H, Wright M, Jonsen M, Varkony T, Jones C, Sage M, Gold S, Morse H, Mendez M, Erickson P, Development of a somatic cell hybrid mapping panel and molecular probes for human chromosome 3. Genomics8:435-46 1990
PubMed ID: 2286371

External Links

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dbSNP dbSNP ID: 11632

Culture Protocols

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Split Ratio 1:4
Temperature 37 C
Percent CO2 5%
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent
Serum 7% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA
Supplement Aminopterin 4 x 10-7M
Supplement Hypoxanthine 1 x 10-4M
Supplement Thymidine 1.6 x 10-5M
Pricing
International/Commercial/For-profit:
$373.00USD
U.S. Academic/Non-profit/Government:
$216.00USD
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How to Order
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