GM11752
Somatic cell hybrid
Description:
CHROMOSOME 3 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 21 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
Human/Chinese hamster hybrid retaining a human der(3)t(3;21) in 96% of cells
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 2p25, D2S1, and 2q37, D2S3. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 3p25, RAF1, and gave a positive result with a probe for 3q26.2qter, TFRC. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 3p14.1-p12, D3S1566, 3p22-p21.33, GLB1, 3p25p24.2, D3S1304, 3q13.1-q13.2, D3S1310, 3q22-q24, D3S1764, 3q26.2-q27, GLUT2, and 3q28-q29, D3S1311. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 21q11, D21S26, and gave a positive result with a probe for 21q22.1q22.2, D21S17. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 21q21.2, APP, 21q21.2, AMYLOID, and 21q22.3, ITGB2, and gave a negative result with a primer for 21p11.2, pAW14a. |
| |
| Remarks |
Line 3;21/UC2-1; produced by the fusion of human lymphoblasts, GM09528, t(3;21) (p24.2;q21), with CHO Q131-91A cells; grown in uridine-def F12 medium with 5% dialyzed FBS; retains der(3)t(3;21) in 96% of cells |
| Chastain-Moore AM, Roberts T, Trott DA, Newbold RF, Ornelles DA, An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression. J Virol77(14):8087-98 2003 |
| PubMed ID: 12829847 |
| |
| Ayyanathan K, Naylor SL, Kunapuli SP, Structural characterization and fine chromosomal mapping of the human P2Y1 purinergic receptor gene (P2RY1). Somat Cell Mol Genet22:419-24 1996 |
| PubMed ID: 9039850 |
| |
| Bailey A, Norris AL, Leek JP, Clissold PM, Carr IM, Ogilvie DJ, Morrison JF, Meredith DM, Markham AF, Yeast artificial chromosome cloning of the beta-catenin locus on human chromosome 3p21-22. Chromosome Res3:201-3 1995 |
| PubMed ID: 7780664 |
| |
| Hess JF, Casselman JT, FitzGerald PG, Chromosomal locations of the genes for the beaded filament proteins CP 115 and CP 47. Curr Eye Res14:11-8 1995 |
| PubMed ID: 7720401 |
| |
| Bardenheuer W, Szymanski S, Lux A, Ludecke HJ, Horsthemke B, Claussen U, Senger G, Smith DI, Wang ND, LePaslier D, et al, Characterization of a microdissection library from human chromosome region 3p14. Genomics19:291-7 1994 |
| PubMed ID: 8188259 |
| |
| Collod G, Babron MC, Jondeau G, Coulon M, Weissenbach J, Dubourg O, Bourdarias JP, Bonaiti-Pellie C, Junien C, Boileau C, A second locus for Marfan syndrome maps to chromosome 3p24.2-p25 [see comments] Nat Genet8:264-8 1994 |
| PubMed ID: 7632217 |
| |
| Naylor SL, Buys CH, Carritt B, Report and abstracts of the Fourth International Workshop on Human Chromosome 3 Mapping Cytogenetics and cell genetics65:2-50 1994 |
| PubMed ID: 8104766 |
| |
| Drabkin H, Wright M, Jonsen M, Varkony T, Jones C, Sage M, Gold S, Morse H, Mendez M, Erickson P, Development of a somatic cell hybrid mapping panel and molecular probes for human chromosome 3. Genomics8:435-46 1990 |
| PubMed ID: 2286371 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 deficient Medium with 2mM L-glutamine or equivalent |
| Serum |
5% dialyzed fetal bovine serum |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 50mg/L |
|
|