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GM11752 Somatic cell hybrid

Description:

CHROMOSOME 3 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 21 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Hybrids
Cell Type Somatic cell hybrid
Transformant Untransformed
Confirmation Clinical summary/Case history
ISCN Human/Chinese hamster hybrid retaining a human der(3)t(3;21) in 96% of cells
Remarks Line 3;21/UC2-1; produced by the fusion of human lymphoblasts, GM09528, t(3;21) (p24.2;q21), with CHO Q131-91A cells; grown in uridine-def F12 medium with 5% dialyzed FBS; retains der(3)t(3;21) in 96% of cells

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 2p25, D2S1, and 2q37, D2S3.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 3p25, RAF1, and gave a positive result with a probe for 3q26.2qter, TFRC. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 3p14.1-p12, D3S1566, 3p22-p21.33, GLB1, 3p25p24.2, D3S1304, 3q13.1-q13.2, D3S1310, 3q22-q24, D3S1764, 3q26.2-q27, GLUT2, and 3q28-q29, D3S1311.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 21q11, D21S26, and gave a positive result with a probe for 21q22.1q22.2, D21S17. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 21q21.2, APP, 21q21.2, AMYLOID, and 21q22.3, ITGB2, and gave a negative result with a primer for 21p11.2, pAW14a.
 

Phenotypic Data

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Remarks Line 3;21/UC2-1; produced by the fusion of human lymphoblasts, GM09528, t(3;21) (p24.2;q21), with CHO Q131-91A cells; grown in uridine-def F12 medium with 5% dialyzed FBS; retains der(3)t(3;21) in 96% of cells

Publications

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Chastain-Moore AM, Roberts T, Trott DA, Newbold RF, Ornelles DA, An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression. J Virol77(14):8087-98 2003
PubMed ID: 12829847
 
Ayyanathan K, Naylor SL, Kunapuli SP, Structural characterization and fine chromosomal mapping of the human P2Y1 purinergic receptor gene (P2RY1). Somat Cell Mol Genet22:419-24 1996
PubMed ID: 9039850
 
Bailey A, Norris AL, Leek JP, Clissold PM, Carr IM, Ogilvie DJ, Morrison JF, Meredith DM, Markham AF, Yeast artificial chromosome cloning of the beta-catenin locus on human chromosome 3p21-22. Chromosome Res3:201-3 1995
PubMed ID: 7780664
 
Hess JF, Casselman JT, FitzGerald PG, Chromosomal locations of the genes for the beaded filament proteins CP 115 and CP 47. Curr Eye Res14:11-8 1995
PubMed ID: 7720401
 
Bardenheuer W, Szymanski S, Lux A, Ludecke HJ, Horsthemke B, Claussen U, Senger G, Smith DI, Wang ND, LePaslier D, et al, Characterization of a microdissection library from human chromosome region 3p14. Genomics19:291-7 1994
PubMed ID: 8188259
 
Collod G, Babron MC, Jondeau G, Coulon M, Weissenbach J, Dubourg O, Bourdarias JP, Bonaiti-Pellie C, Junien C, Boileau C, A second locus for Marfan syndrome maps to chromosome 3p24.2-p25 [see comments] Nat Genet8:264-8 1994
PubMed ID: 7632217
 
Naylor SL, Buys CH, Carritt B, Report and abstracts of the Fourth International Workshop on Human Chromosome 3 Mapping Cytogenetics and cell genetics65:2-50 1994
PubMed ID: 8104766
 
Drabkin H, Wright M, Jonsen M, Varkony T, Jones C, Sage M, Gold S, Morse H, Mendez M, Erickson P, Development of a somatic cell hybrid mapping panel and molecular probes for human chromosome 3. Genomics8:435-46 1990
PubMed ID: 2286371

External Links

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dbSNP dbSNP ID: 11634

Culture Protocols

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Split Ratio 1:5
Temperature 37 C
Percent CO2 5%
Medium Ham's F12 deficient Medium with 2mM L-glutamine or equivalent
Serum 5% dialyzed fetal bovine serum
Substrate None specified
Subcultivation Method trypsin-EDTA
Supplement Proline 50mg/L
Pricing
International/Commercial/For-profit:
$373.00USD
U.S. Academic/Non-profit/Government:
$216.00USD
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