GM12072
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/CHINESE HAMSTER SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Family Member
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12
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Confirmation
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Clinical summary/Case history
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ISCN
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Chinese hamster/human hybrid retaining human chromosome #12
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for Xp22.32 and Yp11.3, MIC2, and gave a negative result with a probe for Xq28, DXS15. S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for Xp22.32, STS, Xq13.3, PGK1, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 1p13, NGFB, and 1q32, REN. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 2p13, TGFA, 2p23, POMC, and 2q13, IL1A. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 3p, MYL3, 3p22-p21.33, GLB1, 3q21.3-q25, ACPP, and 3q26.2-q27, GLUT2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 4p15.3, QDPR, and 4q11-q13, AFP. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 5p14-p12, C9, and 5q13.3-q14, HMGCR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 6p21.3, TNFA, and 6q24-q27, MAS1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 7p12, EGFR, and 7q31.3, CFTR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 8p21-p11.2, LHRH, 8p23-p22, DEF1, and 8q24, TG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p22, IFNA, and 9q22.3-q31, ALDOB. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 10p11.2, ITGB1, and 10q24, PLAU. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 11p13, CAT, and 11q21-q22, CLG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 12p13.2, PRB1, and 12q14q24.1, D12S7. S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 12p13.3-p13.2, F8VWF, and 12q22-q24.2, PAH, and gave a weak positive result with a primer for 12p12.1, KRAS. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 13q14.3, RB1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 14q11.2, NP, and 14q32.1, PI. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q21-q22.2, B2M. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 16p13.3, HBA, and 16q22.1, LCAT. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17p13.1, MYH2. S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 17p13.1, TP53, 17q, GAS, and 17q21.3-q23, MPO. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 18p11.31-p11.22, TYMS. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 19p13.2, LDLR, and 19q13.2, APOC2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 20 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 20pter-p12, PRNP, and 20q12q13.11, ADA. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 21q22.3, ITGB2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
S. L. Naylor, University of Texas, San Antonio, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 22q11.2, IGLC2. |
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| Remarks |
Line E4E; produced by the fusion of human cells with Chinese hamster ovary Gly A mutant cells; grown in HF12D medium with 10% FBS; retains human chromosome #12 in 100% of cells |
| Cuadrado M, Martinez-Pastor B, Murga M, Toledo LI, Gutierrez-Martinez P, Lopez E, Fernandez-Capetillo O, ATM regulates ATR chromatin loading in response to DNA double-strand breaks The Journal of experimental medicine203:297-303 2006 |
| PubMed ID: 16461339 |
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| Hotzel I, Cheevers WP, A maedi-visna virus strain K1514 receptor gene is located in sheep chromosome 3p and the syntenic region of human chromosome 2. J Gen Virol83(Pt 7):1759-64 2002 |
| PubMed ID: 12075096 |
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| Law ML, Tung L, Morse HG, Berger R, Jones C, Cheah KS, Solomon E, The human type II collagen gene (COL2A1) assigned to 12q14.3. Ann Hum Genet50 ( Pt 2):131-7 1986 |
| PubMed ID: 3435042 |
| Split Ratio |
1:25 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 deficient Medium with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 50mg/L |
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