GM12088
Somatic cell hybrid
Description:
CHROMOSOME 18 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Chinese hamster/human hybrid retaining human der(18)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p22, IFNA, 9pter-q12, RLN1, 9q22.3-q31, ALDOB, 9pter-p22, D9S178, 9p12, 1F6, and 9p21, D9S270. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 18p11.31-p11.22, TYMS, and gave positive results with primers for 18q12.1, TTR, 18p11.1-q11.2, D18S44, and a different primer for the same locus. |
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| Remarks |
Line ADJ-40-32; produced by the fusion of human lymphoblasts, der(18)t(9;18) (p13;p11) with TK-deficient Chinese hamster a23 cells; grown in medium with hypoxanthine, methotrexate, & thymidine; 88% of cells retain human 18qter>18p11 |
| van Kessel AG, Straub RE, Silverman GA, Gerken S, Overhauser J, Report and abstracts of the second international workshop on human chromosome 18 mapping. Doorwerth, the Netherlands, July 19-20, 1993. Cytogenet Cell Genet65:142-65 1994 |
| PubMed ID: 8222751 |
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| Markie D, Jones TA, Sheer D, Bodmer WF, A somatic cell hybrid panel for regional mapping of human chromosome 18. Genomics14:431-6 1992 |
| PubMed ID: 1427858 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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