GM12449
Somatic cell hybrid
Description:
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
Human/Chinese hamster hybrid retaining human der(4)t(4;17)(q21.3;q23.2)
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 4p16.2p15.1, D4S20, and 4q21q23, ADH3. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 4p16.3, D4S126, 4p15.3, QDPR, 4q25, EGF, 4q28, FGA, and 4q35, F11, and gave a positive result with a primer for 4q11q13, AFP. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 7p12, EGFR. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 17p13.1, MYH2, and 17q21.3q23, MPO, and gave a positive result with a probe for 17q25, P4HB. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 17p13.1, TP53, 17q21.3-q23, MPO, and 17q, GAS, and gave a positive result with a primer for 17q25-qter, D17S784. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 18q23, MBP. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 21q22.1q22.2, D21S17. |
| |
| Remarks |
Line UMHG-17/3; produced by the fusion of human cells, 46,XY,t(4;17)(q21.1; q24.1), with TK-deficient Chinese hamster a23 cells; selected in HAT medium; retains human der(4)t(4;17) in 88% of cells |
| Hong H, Xu L, Liu J, Jones WD, Su Z, Ning B, Perkins R, Ge W, Miclaus K, Zhang L, Park K, Green B, Han T, Fang H, Lambert CG, Vega SC, Lin SM, Jafari N, Czika W, Wolfinger RD, Goodsaid F, Tong W, Shi L, Technical reproducibility of genotyping SNP arrays used in genome-wide association studies PloS one7:e44483 2012 |
| PubMed ID: 22970228 |
| |
| Plummer SJ, Simmons JA, Adams L, Casey G, Mapping of 228 ESTs and 26 genes into an integrated physical and genetic map of human chromosome 17. Genomics45:140-6 1997 |
| PubMed ID: 9339370 |
| |
| Flejter WL, Watkins M, Abel KJ, Chandrasekharappa SC, Weber BL, Collins FS, Glover TW, Isolation and characterization of somatic cell hybrids with breakpoints spanning 17q22-->q24. Cytogenet Cell Genet64:222-3 1993 |
| PubMed ID: 8404043 |
| Split Ratio |
1:25 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
|
|