GM13157
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 16 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/mouse somatic cell hybrid retaining a human der(13)t(6;13;16)dup(13)(6pter>6p12::13p11>13q21::13q14>13q34::16q22.1>16qter).ish der(13)(wcp6+,D6Z1-,D13Z1+,wcp13+,wcp16+)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line CY4; produced by the fusion of human cells, 46,XY,t(13;16)(q34;q22.1), with APRT deficient mouse A9 cells; grown in medium with alanosine & adenine; the human der(13)t(6;13;16)dup(13) (6pter>6p12::13p11>13q21::13q14>13q34::16q22.1>16qter).ish der(13)(wcp6+,D6Z1-,D13Z1+,wcp13+,wcp16+) was seen in 100% of cells; the derivative chromosome is now different from original published report |
| Callen DF, Doggett NA, Stallings RL, Chen LZ, Whitmore SA, Lane SA, Nancarrow JK, Apostolou S, Thompson AD, Lapsys NM, et al, High-resolution cytogenetic-based physical map of human chromosome 16. Genomics13:1178-85 1992 |
| PubMed ID: 1505951 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Adenine 5 x 10-2mM |
| Supplement |
Azaserine 5 x 10-2mM |
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