GM13258
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/MOUSE SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Family Member
|
22
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
Human/mouse hybrid retaining human chromosome #22
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 3p25, RAF1. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. |
| |
| Remarks |
Line A9+22; produced by the transfer of neo-marked human, fetal, female, lung fibroblast chromosomes into mouse A9 cells by microcell-mediated chromosome transfer; grown in medium with G418; retains human chromosome #22 in 72% of cells |
| Coskun AK, van Maanen M, Nguyen V, Sutton RE, Human chromosome 2 carries a gene required for production of infectious human immunodeficiency virus type 1 Journal of virology80:3406-15 2006 |
| PubMed ID: 16537608 |
| |
| Ning Y, Lovell M, Cooley LD, Pereira-Smith OM, "PCR karyotype" of monochromosomal somatic cell hybrids. Genomics16:758-60 1993 |
| PubMed ID: 8325652 |
| Split Ratio |
1:25 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Geneticin 250ug/ml |
|
|