GM13259
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/MOUSE SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Family Member
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12
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/mouse hybrid retaining human chromosome #12
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 12q14q24.1, D12S7. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 14q32.1q32.3, D14S13. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 16pterp13, D16S36, and 16q13q22.1, MT2. |
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| Remarks |
Line A9+12; produced by the transfer of neo-marked human, fetal, female, lung fibroblast chromosomes into mouse A9 cells by microcell-mediated chromosome transfer; grown in medium with G418; retains human chromosome #12 in 84% of cells |
| Coskun AK, van Maanen M, Nguyen V, Sutton RE, Human chromosome 2 carries a gene required for production of infectious human immunodeficiency virus type 1 Journal of virology80:3406-15 2006 |
| PubMed ID: 16537608 |
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| Cuthbert AP, Bond J, Trott DA, Gill S, Broni J, Marriott A, Khoudoli G, Parkinson EK, Cooper CS, Newbold RF, Telomerase repressor sequences on chromosome 3 and induction of permanent growth arrest in human breast cancer cells [see comments] J Natl Cancer Inst91:37-45 1999 |
| PubMed ID: 9890168 |
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| Ning Y, Lovell M, Cooley LD, Pereira-Smith OM, "PCR karyotype" of monochromosomal somatic cell hybrids. Genomics16:758-60 1993 |
| PubMed ID: 8325652 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Geneticin 250ug/ml |
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