GM13302
Somatic cell hybrid
Description:
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
Human/Chinese hamster hybrid retaining human der(7)t(7;17), #4, 10, 11, 12, 13, 14, & 15
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 7p12, EGFR. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 10p11.2, ITGB1. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 14q32.1, PI. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 17p13.1, TP53. |
| |
| Remarks |
Line UMHG-17/1A; produced by the fusion of human lymphoblasts, 46,XY,t(7;17) (q36;q22), with TK-deficient Chinese hamster a23 cells; selected in HAT medium; retains human der(7) in 100% of cells and other human chromosomes |
| Yoshihashi H, Maeyama K, Kosaki R, Ogata T, Tsukahara M, Goto Y, Hata J, Matsuo N, Smith RJ, Kosaki K, Imprinting of human GRB10 and its mutations in two patients with Russell-Silver syndrome. Am J Hum Genet67(2):476-82 2000 |
| PubMed ID: 10856193 |
| |
| Plummer SJ, Simmons JA, Adams L, Casey G, Mapping of 228 ESTs and 26 genes into an integrated physical and genetic map of human chromosome 17. Genomics45:140-6 1997 |
| PubMed ID: 9339370 |
| |
| Wagner T, Tommerup N, Wirth J, Leffers H, Zimmer J, Back E, Weissenbach J, Scherer G, A somatic cell hybrid panel for distal 17q: GDIA1 maps to 17q25.3. Cytogenet Cell Genet76:172-5 1997 |
| PubMed ID: 9186513 |
| |
| Flejter WL, Watkins M, Abel KJ, Chandrasekharappa SC, Weber BL, Collins FS, Glover TW, Isolation and characterization of somatic cell hybrids with breakpoints spanning 17q22-->q24. Cytogenet Cell Genet64:222-3 1993 |
| PubMed ID: 8404043 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
|
|