GM13341
Somatic cell hybrid
Description:
CHROMOSOME 3 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Mouse/human somatic cell hybrid retaining human der(17)t(3;17), #12, #20, & #21
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 3p22-p21.33, GLB1, and 3p25-p24.2, D3S1304, and gave negative results with primers for 3p14.1-p12, D3S1566, 3q13.1-q13.2, D3S1310, 3q22-q24, D3S1764, 3q26.2-q27, GLUT2, and 3q28q29, D3S1311. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 17p13.1, TP53, and 17q21.3-q23, MPO. |
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| Remarks |
Line GL5; human fibro, GM02808, 46,XX, t(3;17)(3qter>3p21::17p13>17pter;17qter> 17p13::3p21>3pter), fused with mouse fibrosarcoma La-t- cells; grown in HAT medium; retains der(17) in 88% of cells, #12 (12%), #20 (10%), & #21 (92%) |
| Plummer SJ, Simmons JA, Adams L, Casey G, Mapping of 228 ESTs and 26 genes into an integrated physical and genetic map of human chromosome 17. Genomics45:140-6 1997 |
| PubMed ID: 9339370 |
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| Leach RJ, Chinn R, Reus BE, Hayes S, Schantz L, Dubois B, Overhauser J, Ballabio A, Drabkin H, Lewis TB, et al, Regional localization of 188 sequence tagged sites on a somatic cell hybrid mapping panel for human chromosome 3. Genomics24:549-56 1994 |
| PubMed ID: 7713507 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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