GM13534

GM13534 Somatic cell hybrid

Description:

ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 9 SOMATIC CELL HYBRIDS

Affected:

No Data

Sex:

No Data

Age:

No Data

Overview

Repository

NIGMS Human Genetic Cell Repository

Subcollection

Hybrids

Cell Type

Somatic cell hybrid

Transformant

Untransformed

Confirmation

Karyotypic analysis after cell line submission to CCR

ISCN

Human/hamster somatic cell hybrid retaining a human 9q34 inserted in a human marker chromosome & a human #14 in 56% of cells

Remarks

Line GATKY10; produced by the fusion of human lymphoblasts with folylpolyglutamate synthetase-deficient CHO GAT cells; grown in medium with 34.5mg/L proline; retains a segment of 9q34 inserted in a human marker chromosome and chromosome #14 in 56% of cells

Characterizations

IDENTIFICATION OF SPECIES OF ORIGIN

Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis

Phenotypic Data

Remarks

External Links

dbSNP

dbSNP ID: 22029

Culture Protocols

Split Ratio

1:5

Temperature

37 C

Percent CO2

Medium

Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent

Serum

10% fetal bovine serum Not inactivated

Substrate

None specified

Subcultivation Method

trypsin-EDTA

Supplement

Proline 34.5mg/L

Pricing

Commercial/For-profit:

$311.00USD

Academic/Non-profit/Government:

$176.00USD