GM13535
Somatic cell hybrid
Description:
CHROMOSOME 9 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Chinese hamster/human hybrid retaining human del(9), #19, #20, #21, & #22
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 9p24, WI-7297, 9p22, IFNA, 9p21, D9S270, 9p12, 1F6, 9pter-q12, RLN1, 9q13-q21, D9S301, 9q34.3, DBH, and 9q34-qter, D9S158, and gave negative results with primers for 9q22.3-q31, ALDOB, and 9q22.3-q31, D9S53. |
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| Remarks |
Line GAT2806B1; produced by the fusion of human lymphoblasts, 46,XX,del(9)(q22; q33), with folylpolyglutamate synthetase deficient CHO GAT cells; grown in medium with 34.5mg/L proline; retains del(9) in 68% of cells & other human chromosomes |
| Bale, Construction of a somatic cell hybrid mapping panel for chromosome 9q. Cytogenet Cell Genet64:108 (1993): 1993 |
| PubMed ID: |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 34.5mg/L |
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